SAB4200048
Anti-E1B-AP5 antibody, Rat monoclonal
clone EAP4A11, purified from hybridoma cell culture
别名:
Anti-Adenovirus E1B 55-KD protein-associated protein 5, Anti-Heterogeneous nuclear ribonucleoprotein U-like 1, Anti-hnRNPUL1, Anti-hnRPUL1, Monoclonal Anti-E1B-AP5 antibody produced in rat
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
EAP4A11, monoclonal
Application:
ICC, IP, WB
Citations:
4
biological source
rat
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
EAP4A11, monoclonal
form
buffered aqueous solution
mol wt
antigen ~110 kDa
species reactivity
bovine, canine, human, rat, monkey, mouse
technique(s)
immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: 0.05-0.1 μg/mL using HT-29 cell extracts
isotype
IgG2b
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... HNRNPUL1(11100)
General description
Monoclonal Anti-E1B-AP5 (rat IgG2b isotype) is derived from the hybridoma EAP4A11 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with full length human E1B-AP5 fusion protein. E1B-55K-associated protein 5 (E1B-AP5), also known as heterogeneous nuclear ribonucleoprotein U-like 1(hnRNPUL1), is a nuclear RNA-binding protein of the heterogeneous nuclear ribonucleoprotein (hnRNP) family.
The E1B-AP5 gene is located on the human chromosome at 19q13.2.
Immunogen
full length human E1B-AP5 fusion protein.
Application
Monoclonal Anti-E1B-AP5 antibody produced in rat may be used in:
- immunoblotting
- immunoprecipitation
- immunocytochemistry
Biochem/physiol Actions
E1B-55K-associated protein 5 (E1B-AP5) plays a vital role in ATR signaling. In addition, it may play an important role in nucleocytoplasmic RNA transport, and its function is modulated by E1B55kDa in adenovirus-infected cells. Interestingly, this protein was found to be associated with bromodomain-containing protein, BRD7 in a complex that links chromatin events with mRNA processing at the level of transcriptional regulation. It also activates transcription of glucocorticoid-responsive promoter in the absence of ligand-stimulation.
Monoclonal Anti-E1B-AP5 recognizes human, monkey, bovine, dog, mouse and rat E1B-AP5.
Physical form
Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Preparation Note
Store at –20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Regulation of transcription by the heterogeneous nuclear ribonucleoprotein E1B-AP5 is mediated by complex formation with the novel bromodomain-containing protein BRD7.
Kzhyshkowska J, et al.
The Biochemical Journal, 371, 385-385 (2003)
The interaction of the hnRNP family member E1B-AP5 with p53
Barral PM, et al.
Febs Letters, 579, 2752-2758 (2005)
Kentaro Ohki et al.
Haematologica, 104(1), 128-137 (2018-09-02)
Fusion genes involving MEF2D have recently been identified in precursor B-cell acute lymphoblastic leukemia, mutually exclusive of the common risk stratifying genetic abnormalities, although their true incidence and associated clinical characteristics remain unknown. We identified 16 cases of acute lymphoblastic
Regulation of DNA-End Resection by hnRNPU-like Proteins Promotes DNA Double-Strand Break Signaling and Repair
Polo SE, et al.
Molecular Cell, 45, 505-516 (2012)
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