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Merck
CN

SAB4200065

Anti-EIF2C1/Ago1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

别名:

Anti-Argonaute-1, Anti-Eukaryotic translation initiation factor 2C, subunit 1, Anti-GERP95, Anti-Golgi Endoplasmic Reticulum protein 95 kDa

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UNSPSC Code:
12352203

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biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~100 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using lysates of HEK-293T cells over expressing hEIF2C1/Ago1, indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde fixed HEK-293T cells over expressing hEIF2C1/Ago1, western blot: 2-4 μg/mL using lysates of HEK-293T cells over expressing hEIF2C1/Ago1

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... EIF2C1(26523)
mouse ... Eif2c1(236511)
rat ... Eif2c1(313594)

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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存储类别

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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分析证书(COA)

Lot/Batch Number
010M4774

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Anumeha Singh et al.
The EMBO journal, 38(16), e100727-e100727 (2019-07-23)
Translational readthrough generates proteins with extended C-termini, which often possess distinct properties. Here, we have used various reporter assays to demonstrate translational readthrough of AGO1 mRNA. Analysis of ribosome profiling data and mass spectrometry data provided additional evidence for translational
Tingfang Yi et al.
Nature communications, 6, 7194-7194 (2015-05-29)
MicroRNA (miRNA) biogenesis and miRNA-guided RNA interference (RNAi) are essential for gene expression in eukaryotes. Here we report that translation initiation factor eIF1A directly interacts with Ago2 and promotes Ago2 activities in RNAi and miR-451 biogenesis. Biochemical and NMR analyses

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