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Merck
CN

SAB4200066

Sigma-Aldrich

Anti-EIF2C1/Ago1 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

别名:

Anti-Argonaute-1, Anti-Eukaryotic translation initiation factor 2C, subunit 1, Anti-GERP95, Anti-Golgi Endoplasmic Reticulum protein 95 kDa

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关于此项目

UNSPSC代码:
12352203
NACRES:
NA.41

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生物来源

rabbit

偶联物

unconjugated

抗体形式

affinity isolated antibody

抗体产品类型

primary antibodies

克隆

polyclonal

表单

buffered aqueous solution

分子量

antigen ~100 kDa

种属反应性

human

浓度

~1.0 mg/mL

技术

immunoprecipitation (IP): 5-10 μg using lysates of HEK-293T cells over expressing hEIF2C1/Ago1
indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde fixed HEK-293T cells over expressing hEIF2C1/Ago1
western blot: 0.5-1 μg/mL using lysates of HEK-293T cells over expressing hEIF2C1/Ago1

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

基因信息

human ... EIF2C1(26523)
mouse ... Eif2c1(236511)
rat ... Eif2c1(313594)

一般描述

In humans, the argonaute (Ago) subfamily consists of four isoforms hsAgo1−4. Ago proteins (~100 kDa molecular weight) contain a piwi-argonaute-zwille (PAZ) and PIWI domains. These proteins are evolutionarily conserved between species. EIF2C1/Ago1 is predominantly expressed in embryonic kidney and lung.
The eukaryotic initiation factor (EIF2C1), also known as argonaute 1, RNA-induced silencing complex (RISC) catalytic component, is encoded by the gene with 19 exons and 18 introns spanning ~ 50 kb on genomic DNA, mapped to human chromosome 1p34.3. The gene is widely expressed at low and medium levels. The encoded protein is a member of argonaute family of proteins.

应用

Anti-EIF2C1/Ago1 (N-terminal) antibody produced in rabbit has also been used in:
  • immunoblotting
  • immunoprecipitation
  • immunofluorescence

Anti-EIF2C1/Ago1 (N-terminal) antibody produced in rabbit has been used in chromatin immunoprecipitation (ChIP) and RNA-native ChIP (NChIP) experiments.

生化/生理作用

EIF2C1 is implicated in both transcriptional and post-transcriptional gene silencing. EIF2C1 levels are also increased in tumors that lack the Wilm′s tumor suppressor gene, WT1.
Eukaryotic initiation factor (EIF2C1)/ argonaute 1 plays a vital role in activation of transcriptional enhancers and also regulates alternative splicing in human cells. The encoded protein interacts with hypoxia-responsive microRNAs (HRMs) and stimulate angiogenic pathway.

外形

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

10 - Combustible liquids

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
068M4831V
010M4773

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Argonaute proteins at a glance
Ender C and Meister G
Journal of Cell Science, 123(11), 1819-1823 (2010)
Argonaute proteins couple chromatin silencing to alternative splicing.
Ameyar-Zazoua M
Nature Structural and Molecular Biology, 19, 998-1004 (2012)
Argonaute proteins: potential biomarkers for human colon cancer
Li L, et al.
BMC Cancer, 10(1), 38-38 (2010)
Hypoxia-responsive miRNAs target argonaute 1 to promote angiogenesis
Chen Z
The Journal of Clinical Investigation, 123, 1057-1067 (2013)
The making of a slicer: activation of human Argonaute-1.
Faehnle CR
Cell Reports, 3(6), 1901-1909 (2013)
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