SAB4200090
Anti-Raver1 (C-terminal) antibody produced in rabbit
IgA fraction of antiserum
别名:
Anti-Ribonucleoprotein PTB-binding 1
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, IP, WB
Citations:
3
biological source
rabbit
conjugate
unconjugated
antibody form
IgA fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~77 kDa
technique(s)
immunoprecipitation (IP): 5-10 μL using lysates of HEK-293Tcells overexpressing human Raver1, indirect immunofluorescence: 1:500-1:1000 using paraformaldehyde fixed HEK-293T cells overexpressing human Raver1, western blot: 1:500-1:1000 using lysates of HEK-293T cells overexpressing human Raver1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... RAVER1(125950)
mouse ... Raver1(71766)
rat ... Raver1(298705)
General description
Raver1 is encoded by the gene mapped to human chromosome 19p13.2.
Raver1, also known as ribonucleoprotein phosphotyrosine-binding (PTB)-1, is a widely expressed multidomain protein. Raver1 is composed of three RNA recognition motifs (RRM) and both nuclear localization and nuclear export signals. It thus, shuttles between the nucleus and the cytoplasm. Raver1 also colocalizes with PTB/hnRNPI, a protein involved in RNA splicing of microfilament proteins.
Application
Anti-Raver1 (C-terminal) antibody produced in rabbit is suitable for:
- immunoblotting
- immunoprecipitation
- immunofluorescence
Biochem/physiol Actions
Anti-Raver1 (C-terminal) recognizes human Raver1.
Variation in the raver1 gene expression may be associated with the development of depressive disorder. In skeletal muscle, migration of of Raver1 from the nucleus to the cytoplasm facilitates differentiation of specific microfilament attachment sites.
Vinculin recruits raver1 and its mRNAs cargo to focal adhesions, promoting localization of the synthesis of adhesion complexes by the translational machinery.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Hee-Ju Kang et al.
Scientific reports, 10(1), 9927-9927 (2020-06-20)
The prevalence and clinical characteristics of depressive disorders differ between women and men; however, the genetic contribution to sex differences in depressive disorders has not been elucidated. To evaluate sex-specific differences in the genetic architecture of depression, whole exome sequencing
Raver1, a dual compartment protein, is a ligand for PTB/hnRNPI and microfilament attachment proteins
Huttelmaier S, et al.
The Journal of Cell Biology, 155(5), 775-786 (2001)
Raver1 interactions with vinculin and RNA suggest a feed-forward pathway in directing mRNA to focal adhesions
Lee JH, et al.
Structure, 17(6), 833-842 (2009)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| SAB4200090-200UL | 04061835686933 |
| SAB4200090-25UL | 04061835686940 |