Anti-DOCK9 (Zizimin 1) antibody, Mouse monoclonal

Monoclonal Anti-DOCK9 (Zizimin1) (mouse IgG2a isotype) is derived from the hybridoma 8B3-C3-E4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Zizimin1 is a guanine nucleotide exchange factor (GEF) specific to Cdc42. It is a part of the second family of mammalian Rho-GEFs, CZH [CDM (Ced-5/DOCK180/Myoblast city)-zizimin homology] proteins. The Zizimin1 protein contains the CZH2 domain, CZH1 domain and Pleckstrin homology (PH) domain.

full length DOCK9 (Zizimin1)

Anti-DOCK9 (Zizimin 1) antibody, Mouse monoclonal is suitable for:

• immunoblotting
• immunoprecipitation
• immunocytochemistry
• immunohistochemistry

Monoclonal Anti-DOCK9 (Zizimin1) recognizes human DOCK9.

Zizimin1/ dedicator of cytokinesis 9 (DOCK9) is found to activate Cdc42 through binding to its CZH2 domain. Interestingly, the CZH1 domain of Zizimin1 at the N-terminal binds to its CZH2 domain and thus inhibits Cdc42 binding. The Pleckstrin homology (PH) domain at the N-terminal, binds phosphoinositides and results in zizimin1 membrane targeting.

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Store at -20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, freeze in working aliquots at -20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

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