SAB4200093
Anti-DOCK9 (Zizimin 1) antibody, Mouse monoclonal
clone 8B3-C3-E4, purified from hybridoma cell culture
别名:
Anti-Dedicator of cytokinesis 9, Anti-ZIZ1
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
8B3-C3-E4, monoclonal
Application:
ICC, IHC, IP, WB
Citations:
2
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
8B3-C3-E4, monoclonal
form
buffered aqueous solution
mol wt
antigen ~236 kDa
species reactivity
human
packaging
antibody small pack of 25 μL
concentration
~1.0 mg/mL
technique(s)
immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: 3.0-6.0 μg/mL using MDA-MB-231 cell extracts
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... DOCK9(23348)
General description
Monoclonal Anti-DOCK9 (Zizimin1) (mouse IgG2a isotype) is derived from the hybridoma 8B3-C3-E4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Zizimin1 is a guanine nucleotide exchange factor (GEF) specific to Cdc42. It is a part of the second family of mammalian Rho-GEFs, CZH [CDM (Ced-5/DOCK180/Myoblast city)-zizimin homology] proteins. The Zizimin1 protein contains the CZH2 domain, CZH1 domain and Pleckstrin homology (PH) domain.
Immunogen
full length DOCK9 (Zizimin1)
Application
Anti-DOCK9 (Zizimin 1) antibody, Mouse monoclonal is suitable for:
- immunoblotting
- immunoprecipitation
- immunocytochemistry
- immunohistochemistry
Biochem/physiol Actions
Monoclonal Anti-DOCK9 (Zizimin1) recognizes human DOCK9.
Zizimin1/ dedicator of cytokinesis 9 (DOCK9) is found to activate Cdc42 through binding to its CZH2 domain. Interestingly, the CZH1 domain of Zizimin1 at the N-terminal binds to its CZH2 domain and thus inhibits Cdc42 binding. The Pleckstrin homology (PH) domain at the N-terminal, binds phosphoinositides and results in zizimin1 membrane targeting.
Physical form
Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Preparation Note
Store at -20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, freeze in working aliquots at -20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
The novel Cdc42 guanine nucleotide exchange factor, zizimin1, dimerizes via the Cdc42-binding CZH2 domain
Meller N, et al.
Test, 279(36), 37470-37476 (2004)
Function of the N-terminus of zizimin1: autoinhibition and membrane targeting
Meller N, et al.
The Biochemical Journal, 409(2), 525-533 (2008)
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