SAB4200110
Anti-PIWIL1 (internal region) antibody produced in rabbit
~1.0 mg/mL, buffered aqueous solution
别名:
Anti-HIWI, Anti-MIWI, Anti-PIWI-like 1
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IHC, WB
Citations:
4
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
~95 kDa
species reactivity
human, rat, mouse
concentration
~1.0 mg/mL
technique(s)
immunohistochemistry: suitable using heat-retrieved formalin-fixed, paraffin-embedded human colon carcinoma sections and biotin/ExtrAvidin®-Peroxidase staining system, western blot: 1-2 μg/mL using rat and mouse testis extracts
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... PIWIL1(9271)
mouse ... Piwil1(57749)
rat ... Piwil1(363912)
General description
The PIWIL1 (Piwi like RNA-mediated gene silencing 1) gene encodes an 861 amino acid protein, which belongs to the Piwi subfamily of Argonaute proteins. This gene is mapped to human chromosome 12q24.33. The Piwi proteins are mostly localized to the germline, where they bind to PIWI-interacting RNAs (piRNAs). The Piwi proteins contain the Piwi-Argonaute-Zwille (PAZ) domain and the PIWI domain.
Application
Anti-PIWIL1 (internal region) antibody produced in rabbit is suitable for immunoblotting and immunohistochemistry.
Biochem/physiol Actions
Anti-PIWIL1 (internal region) recognizes human, mouse, and rat PIWIL1.
The Piwi proteins are important for the biogenesis and function of small non-coding RNAs. These proteins and PIWI-interacting RNAs (piRNAs) have been implicated in epigenetic control of gene expression, transposon silencing, gene expression, and translation regulation. The Piwi proteins significantly participate in germline development and gametogenesis of various metazoan species. These proteins undergo symmetrical dimethyl arginines (sDMAs) modification by protein methyltransferase 5 (PRMT5).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Preparation Note
Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation. Working dilutions should be discarded if not used within 12 hours.
Legal Information
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Arginine methylation of Piwi proteins catalysed by dPRMT5 is required for Ago3 and Aub stability
Kirino Y, et al.
Nature Cell Biology, 11(5) (2009)
The Biogenesis and Function PIWI Proteins and piRNAs: Progress and Prospect
Thomson T and Lin H
Annual Review of Cell and Developmental Biology, ,
number=(25), 355?376-355?376 (2009)
Expression status of PIWIL1 as a prognostic marker of colorectal cancer
Sun R, et al.
Disease Markers, 2017(5) (2017)
The Argonaute protein family
Hock J and Gunter M
Genome Biology, 9(2), 210-210 (2008)
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