生物来源
rabbit
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
~95 kDa
种属反应性
human, rat, mouse
浓度
~1.0 mg/mL
技术
immunohistochemistry: suitable using heat-retrieved formalin-fixed, paraffin-embedded human colon carcinoma sections and biotin/ExtrAvidin®-Peroxidase staining system
western blot: 1-2 μg/mL using rat and mouse testis extracts
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... PIWIL1(9271)
mouse ... Piwil1(57749)
rat ... Piwil1(363912)
一般描述
The PIWIL1 (Piwi like RNA-mediated gene silencing 1) gene encodes an 861 amino acid protein, which belongs to the Piwi subfamily of Argonaute proteins. This gene is mapped to human chromosome 12q24.33. The Piwi proteins are mostly localized to the germline, where they bind to PIWI-interacting RNAs (piRNAs). The Piwi proteins contain the Piwi-Argonaute-Zwille (PAZ) domain and the PIWI domain.
应用
Anti-PIWIL1 (internal region) antibody produced in rabbit is suitable for immunoblotting and immunohistochemistry.
生化/生理作用
Anti-PIWIL1 (internal region) recognizes human, mouse, and rat PIWIL1.
The Piwi proteins are important for the biogenesis and function of small non-coding RNAs. These proteins and PIWI-interacting RNAs (piRNAs) have been implicated in epigenetic control of gene expression, transposon silencing, gene expression, and translation regulation. The Piwi proteins significantly participate in germline development and gametogenesis of various metazoan species. These proteins undergo symmetrical dimethyl arginines (sDMAs) modification by protein methyltransferase 5 (PRMT5).
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
制备说明
Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation. Working dilutions should be discarded if not used within 12 hours.
法律信息
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
此项目有
历史批次信息供参考:
Arginine methylation of Piwi proteins catalysed by dPRMT5 is required for Ago3 and Aub stability
Kirino Y, et al.
Nature Cell Biology, 11(5) (2009)
Expression status of PIWIL1 as a prognostic marker of colorectal cancer
Sun R, et al.
Disease Markers, 2017(5) (2017)
The Biogenesis and Function PIWI Proteins and piRNAs: Progress and Prospect
Thomson T and Lin H
Annual Review of Cell and Developmental Biology, ,
number=(25), 355?376-355?376 (2009)
The Argonaute protein family
Hock J and Gunter M
Genome Biology, 9(2), 210-210 (2008)
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