SAB4200114
Anti-EDC4 (N-terminal) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody
别名:
Anti-Ge-1, Anti-HEDLS (Human enhancer of decapping, (large subunit), Anti-RCD-8
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, IP, WB
Citations:
9
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~150 kDa
species reactivity
human
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): 5-10 μg using ysates of HEK-293 cells over expressing human EDC4, indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde fixed NIH-3T3 cells over expressing human EDC4, western blot: 0.5-1 μg/mL using lysates of HEK-293 cells over expressing human EDC4
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... EDC4(23644)
mouse ... Edc4(234699)
rat ... Edc4(361399)
General description
Enhancer of mRNA-decapping protein 4 (EDC4), also known as human enhancer of decapping large subunit (HEDLS) or Ge-1, is characterized with an N-terminal WD40 repeat and a C-terminal domain which interacts with mRNA-decapping enzyme subunit 2 (Dcp2), and mediates EDC4 oligomerization and P-body localization .
The EDC4 gene maps on human chromosome 16q22.1.
Application
Anti-EDC4 (N-terminal) antibody has been used in:
- immunoprecipitation
- immunofluorescence
- immunoblotting.
Biochem/physiol Actions
Anti-EDC4 (N-terminal) specifically recognizes human EDC4.
Human enhancer of mRNA-decapping protein 4 (EDC4) associates with hDcp2 and stimulates hDcp2 activity in vitro as well as mediating the interaction between mRNA-decapping enzyme subunit 1 & 2 (DCP1 and DCP2).
Interaction of processing-body components, EDC4 and Dcp1a is essential for the post-transcriptional regulation of interleukin (IL)-6.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
常规特殊物品
此项目有
The Assembly of EDC4 and Dcp1a into Processing Bodies Is Critical for the Translational Regulation of IL-6
Seto E, et al.
PLoS ONE, 10(5) (2015)
A human microprotein that interacts with the mRNA decapping complex
D'Lima N G, et al.
Nature Chemical Biology, 13(2), 174-174 (2017)
Target-specific requirements for enhancers of decapping in miRNA-mediated gene silencing
Eulalio A, et al.
Genes & Development, 21(20), 2558-2570 (2007)
Jucimara Colombo et al.
Oncology reports, 21(3), 649-663 (2009-02-13)
Laryngeal squamous cell carcinoma is very common in head and neck cancer, with high mortality rates and poor prognosis. In this study, we compared expression profiles of clinical samples from 13 larynx tumors and 10 non-neoplastic larynx tissues using a
Denis Chang et al.
The Journal of cell biology, 220(9) (2021-07-13)
Epithelial cells lining mucosal surfaces distinctively express the inflammatory bowel disease risk gene INAVA. We previously found that INAVA has dual and competing functions: one at lateral membranes where it affects mucosal barrier function and the other in the cytosol
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