SAB4200134
Anti-PNK antibody ,Mouse monoclonal
clone H101.5, purified from hybridoma cell culture
别名:
Anti-Bifunctional polynucleotide phosphatase/kinase, Anti-DNA 5′-kinase/3′-phosphatase, Anti-PNKP, Anti-Polynucleotide kinase 3′-phosphatase
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关于此项目
NACRES:
NA.44
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
H101.5, monoclonal
Application:
WB
Citations:
7
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
H101.5, monoclonal
form
buffered aqueous solution
mol wt
antigen ~55 kDa
species reactivity
monkey, human, bovine
packaging
antibody small pack of 25 μL
concentration
~1.0 mg/mL
technique(s)
western blot: 2.5-5.0 μg/mL using whole extracts of A549 cells
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... PNKP(11284)
General description
Monoclonal Anti-PNK (mouse IgG1 isotype) is derived from the hybridoma H101.5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a human polynucleotide kinase 3′-phosphatase (PNK) recombinant protein. PNK comprises of a central phosphatase domain that is flanked by an N-terminal forkhead-associated (FHA) domain and a C-terminal kinase domain on either sides.
Immunogen
human PNK recombinant protein.
Application
Monoclonal Anti-PNK antibody produced in mouse has been used in immunoblotting and immunoprecipitation.
Biochem/physiol Actions
PNK is a mammalian DNA repair enzyme which catalyzes the phosphorylation of DNA 5′-hydroxyl termini and the dephosphorylation of 3′-phosphate termini. PNK is involved in the single-strand break repair pathway and the non-homologous end joining pathway for double strand break repair. Mutations in PNK result in neurological abnormalities.
Polynucleotide kinase 3′-phosphatase (PNKP or PNK) is a nuclear protein that repairs DNA strand breaks induced by oxidative damage or ionizing radiation. Genetic alterations in PNKP have been linked to seizures, microcephaly and deficiencies in DNA repair mechanisms .
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Production, characterization, and epitope mapping of monoclonal antibodies against human polydeoxyribonucleotide kinase
Fanta M, et al.
Hybridoma, 20(4), 237-242 (2001)
Human polynucleotide kinase participates in repair of DNA double-strand breaks by nonhomologous end joining but not homologous recombination
Karimi BF, et al.
Cancer research, 67(14), 6619-6625 (2007)
PNKP knockdown by RNA interference inhibits herpes simplex virus-1 replication in astrocytes
Yue L, et al.
Virologica Sinica, 28(6), 345-351 (2013)
F Karimi-Busheri et al.
The Journal of biological chemistry, 274(34), 24187-24194 (1999-08-14)
Human polydeoxyribonucleotide kinase is an enzyme that has the capacity to phosphorylate DNA at 5'-hydroxyl termini and dephosphorylate 3'-phosphate termini and, therefore, can be considered a putative DNA repair enzyme. The enzyme was purified from HeLa cells. Amino acid sequence
N K Bernstein et al.
Anti-cancer agents in medicinal chemistry, 8(4), 358-367 (2008-05-14)
The cytotoxicity of many antineoplastic agents is due to their capacity to damage DNA and there is evidence indicating that DNA repair contributes to the cellular resistance to such agents. DNA strand breaks constitute a significant proportion of the lesions
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