SAB4200172
Anti-NEZHA (N-terminal) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody
别名:
Anti-3 calmodulin-regulated spectrin-associated protein 3, Anti-KIAA154
生物来源
rabbit
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen ~150 kDa
种属反应性
human
浓度
~1.0 mg/mL
技术
immunoprecipitation (IP): 2.5-5.0 μg using lysates of HEK-293T cells overexpressing human NEZHA.
western blot: 1-2 μg/mL using lysates of HEK-293T cells overexpressing human NEZHA.
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... KIAA1543(57662)
mouse ... 2310057J16Rik(69697)
rat ... Camsap3(689074)
一般描述
Nezha (a deity in Chinese mythology), also known as KIAA1543 and calmodulin-regulated spectrin associated protein 3 (CAMSAP3) contains one calponin homology (CH) and two coiled coil (CC1 and 2) domains, as well as one DUF1781 (DUF) domain of unknown function. Nezha exists in the cells in two pools: junctional and cytoplasmic. This protein is known to be localized in the noncentrosomal microtubules. The NEZHA gene is mapped to human chromosome 19p13.2.
免疫原
synthetic peptide corresponding to the N- terminal region of human NEZHA, conjugated to KLH. The corresponding sequence is identical in mouse and rat.
应用
Anti-NEZHA (N-terminal) antibody produced in rabbit is suitable for immunoblotting and immunoprecipitation.
生化/生理作用
Anti-NEZHA (N-terminal) recognizes human NEZHA.
Nezha and its binding partner Pleckstrin homology domain containing A7 (PLEKHA7) are discovered as new proteins which are part of the cadherin-based protein complex in mammalian epithelial cells. This complex can anchor microtubule minus ends to the zonula adherens (ZA) and is necessary for the biogenesis of this specialized junction. The junctional Nezha is recruited to this site via its interaction with PLEKHA7. Reduction of Nezha is shown to disrupt the ZA, in vitro.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
制备说明
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, orstorage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
未找到合适的产品？
试试我们的产品选型工具.
储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
此项目有
Jennifer B Permuth et al.
Human molecular genetics, 25(16), 3600-3612 (2016-07-06)
Rare and low frequency variants are not well covered in most germline genotyping arrays and are understudied in relation to epithelial ovarian cancer (EOC) risk. To address this gap, we used genotyping arrays targeting rarer protein-coding variation in 8,165 EOC
Anna Akhmanova et al.
Cell, 135(5), 791-793 (2008-12-02)
The zonula adherens (ZA) is a specialized cadherin-based structure found at the contacts between epithelial cells. Meng et al. (2008) now identify a protein complex containing the microtubule minus-end-binding protein Nezha, which provides a critical link between microtubules and cadherins
Wenxiang Meng et al.
Cell, 135(5), 948-959 (2008-12-02)
Epithelial cells contain noncentrosomal microtubules (MTs), whose minus ends are oriented apically. In contrast with the well-known interactions of the minus ends with the centrosome, little is known about the termination site of the noncentrosomal minus ends. Here we show
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持