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Merck
CN

SAB4200232

Sigma-Aldrich

Anti-GAD65 Antibody

mouse monoclonal, N-GAD65

别名:

Anti-GAD2, Anti-glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa), Anti-glutamate decarboxylase 65 kDa isoform

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关于此项目

MDL编号:
MFCD03455043
UNSPSC代码:
12352203
NACRES:
NA.41

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产品名称

Anti-GAD65 antibody , Mouse monoclonal, clone N-GAD65, purified from hybridoma cell culture

生物来源

mouse

质量水平

200

偶联物

unconjugated

抗体形式

purified from hybridoma cell culture

抗体产品类型

primary antibodies

克隆

N-GAD65, monoclonal

表单

buffered aqueous solution

分子量

antigen ~65 kDa

种属反应性

human, mouse, monkey, rat

浓度

~1.0 mg/mL

技术

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 0.25-0.5 μg/mL using whole extracts of rat brain

同位素/亚型

IgG1

UniProt登记号

Q05329

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

基因信息

human ... GAD2(2572)
mouse ... Gad2(14417)
rat ... Gad2(24380)

相关类别

一般描述

Glutamate decarboxylase 2 (GAD2) encodes GAD65, an isoform of glutamate decarboxylase. GAD65 is majorly expressed in axon terminal regions in neurons. GAD65 is present in the synaptic vesicles. GAD2 is mapped to human chromosome 10p12.1. It has an N-terminal pyridoxal 5′-phosphate (PLP) binding −domain.

应用

Monoclonal Anti-GAD65 antibody produced in mouse has been used in the detection of recombinant GAD65 using ELISA.

生化/生理作用

Glutamate decarboxylase 2 (GAD2) protein GAD65 catalyzes the formation of γ-aminobutyric acid (GABA) from glutamic acid. Palmitoylation of GAD65 is crucial for protein sorting in synaptic clusters. GAD65 autoantigen is prevalent in type 1 diabetes. Its expression increases on allergen exposure. GAD65, along with γ-aminobutyric acid (GABA), may play a key role in the pathogenesis of asthma.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000395464
0000365039
0000310386
0000295321
0000365039

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Purification and characterization of GAD65-specific monoclonal autoantibodies [version 1; referees: awaiting peer review]
Jiang W, et al.
F1000Research, 5003(7), 1VL-1VL (2015)
GAD65 autoantibodies and its role as biomarker of Type 1 diabetes and Latent Autoimmune Diabetes in Adults (LADA)
Towns R and Pietropaolo M
Drugs of the Future, 36(11), 847-847 (2011)
Wei Jiang et al.
F1000Research, 4, 135-135 (2015-05-29)
Autoantibodies against antigens expressed by insulin-producing β cells are circulating in both healthy individuals and patients at risk of developing Type 1 diabetes. Recent studies suggest that another set of antibodies (anti-idiotypic antibodies) exists in this antibody/antigen interacting network to regulate
A GABAergic system in airway epithelium is essential for mucus overproduction in asthma
Xiang YY, et al.
Nature Medicine, 13(7), 862-862 (2007)
A combination of three distinct trafficking signals mediates axonal targeting and presynaptic clustering of GAD65
Kanaani J, et al.
The Journal of Cell Biology, 158(7), 1229-1238 (2002)
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