SAB4200232
Anti-GAD65 Antibody
mouse monoclonal, N-GAD65
别名:
Anti-GAD2, Anti-glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa), Anti-glutamate decarboxylase 65 kDa isoform
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关于此项目
Conjugate:
unconjugated
Clone:
N-GAD65, monoclonal
Application:
immunohistochemistry
immunoprecipitation (IP)
western blot
immunoprecipitation (IP)
western blot
Species reactivity:
human, mouse, monkey, rat
Citations:
12
Technique(s):
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 0.25-0.5 μg/mL using whole extracts of rat brain
immunoprecipitation (IP): suitable
western blot: 0.25-0.5 μg/mL using whole extracts of rat brain
Uniprot accession no.:
产品名称
Anti-GAD65 antibody , Mouse monoclonal, clone N-GAD65, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
N-GAD65, monoclonal
form
buffered aqueous solution
mol wt
antigen ~65 kDa
species reactivity
human, mouse, monkey, rat
concentration
~1.0 mg/mL
technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 0.25-0.5 μg/mL using whole extracts of rat brain
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... GAD2(2572)
mouse ... Gad2(14417)
rat ... Gad2(24380)
Application
Monoclonal Anti-GAD65 antibody produced in mouse has been used in the detection of recombinant GAD65 using ELISA.
Biochem/physiol Actions
Glutamate decarboxylase 2 (GAD2) protein GAD65 catalyzes the formation of γ-aminobutyric acid (GABA) from glutamic acid. Palmitoylation of GAD65 is crucial for protein sorting in synaptic clusters. GAD65 autoantigen is prevalent in type 1 diabetes. Its expression increases on allergen exposure. GAD65, along with γ-aminobutyric acid (GABA), may play a key role in the pathogenesis of asthma.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Glutamate decarboxylase 2 (GAD2) encodes GAD65, an isoform of glutamate decarboxylase. GAD65 is majorly expressed in axon terminal regions in neurons. GAD65 is present in the synaptic vesicles. GAD2 is mapped to human chromosome 10p12.1. It has an N-terminal pyridoxal 5′-phosphate (PLP) binding −domain.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
GAD65 autoantibodies and its role as biomarker of Type 1 diabetes and Latent Autoimmune Diabetes in Adults (LADA)
Towns R and Pietropaolo M
Drugs of the Future, 36(11), 847-847 (2011)
Purification and characterization of GAD65-specific monoclonal autoantibodies [version 1; referees: awaiting peer review]
Jiang W, et al.
F1000Research, 5003(7), 1VL-1VL (2015)
Wei Jiang et al.
F1000Research, 4, 135-135 (2015-05-29)
Autoantibodies against antigens expressed by insulin-producing β cells are circulating in both healthy individuals and patients at risk of developing Type 1 diabetes. Recent studies suggest that another set of antibodies (anti-idiotypic antibodies) exists in this antibody/antigen interacting network to regulate
Optimized purification strategies for the elimination of non-specific products in the isolation of GAD65-specific monoclonal autoantibodies
Jiang W, et al.
F1000Research, 4 (2015)
A GABAergic system in airway epithelium is essential for mucus overproduction in asthma
Xiang YY, et al.
Nature Medicine, 13(7), 862-862 (2007)
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