SAB4200271
Anti-EDEM1(internal) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody
别名:
Anti-ER degradation enhancer, Anti-mannosidase alpha-like 1
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IP, WB
Citations:
3
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 75/65 kDa
species reactivity
human, mouse
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): 2.5-5.0 μg using lysates of HEK-293T cells over expressing mouse EDEM1., western blot: 2-4 μg/mL using whole extracts of human Jurkat cells.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... EDEM1(9695)
mouse ... Edem1(192193)
General description
ER degradation-enhancing α-mannosidase like protein 1 (EDEM1) is a putative mannose-binding lectin. EDEM1 is localized to the endoplasmic reticulum (ER) mainly as a soluble glycoprotein.
Immunogen
synthetic peptide corresponding to an internal region within human EDEM1, conjugated to KLH. The corresponding sequence differs by a single amino acid in mouse and by 2 amino acids in rat.
Application
Anti-EDEM1(internal) antibody produced in rabbit has been used in immunoblotting and immunoprecipitation.
Biochem/physiol Actions
Over-expression of ER degradation-enhancing α-mannosidase like protein 1 (EDEM1) leads to endoplasmic reticulum (ER)-associated degradation (ERAD) by stimulating the release of terminally misfolded glycoproteins from calnexin. EDEM degrades misfolded glycoproteins in an N-glycan dependent manner. Three EDEM homologues, EDEM1, EDEM2 and EDEM3 are transcriptionally upregulated by activated inositol-requiring enzyme 1 (IRE1)/X-box binding protein 1 (XBP1) branch. It interacts with the COOH-terminus of calnexin and lacks mannosidase activity.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
A novel ER alpha-mannosidase-like protein accelerates ER-associated degradation
Hosokawa N, et al.
EMBO Reports, 2(5), 415-422 (2001)
Human EDEM2, a novel homolog of family 47 glycosidases, is involved in ER-associated degradation of glycoproteins
Mast SW, et al.
Glycobiology, 15(4), 421-436 (2004)
Min Ni et al.
FEBS letters, 581(19), 3641-3651 (2007-05-08)
The field of endoplasmic reticulum (ER) stress in mammalian cells has expanded rapidly during the past decade, contributing to understanding of the molecular pathways that allow cells to adapt to perturbations in ER homeostasis. One major mechanism is mediated by
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