SAB4200277
Anti-RCC1 (N-terminal) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody
别名:
Anti-CHC-1, Anti-CHC1, Anti-Chromosome Condensation 1, Anti-Regulator of Chromatin Condensation 1
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, IP, WB
Citations:
2
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
~45 kDa
species reactivity
human
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): 2.5-5.0 μg using lysates of human HEK-293 cells., indirect immunofluorescence: 1.0-2.0 μg/mL using paraformaldehyde fixed HeLa cells., western blot: 0.5-1.0 μg/mL using lysates of HeLa cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... RCC1(1104)
General description
Regulator of chromosome condensation 1 (RCC1) is a nuclear chromatin-bound protein and a guanine nucleotide exchange factor (GEF) for the nuclear Ras-related nuclear (Ran) protein. It consists of seven homologous repeats of 51−68 amino acid residues termed RCC1-like domains (RLDs), which adopt a seven-bladed β-propeller fold 2 and an amino terminal tail extension. The RCC1 gene is mapped to human chromosome 1p35.3.(4)
Immunogen
synthetic peptide corresponding to a sequence at the N- terminal region of human RCC1, conjugated to KLH. The corresponding sequence differs by one amino acid in rat or mouse.
Application
Anti-RCC1 (N-terminal) antibody produced in rabbit is suitable for:
- immunoblotting
- immunofluorescence
- immunoprecipitation
Biochem/physiol Actions
Anti-RCC1 (N-terminal) recognizes human RCC1.
Regulator of chromosome condensation 1 (RCC1) protein is an important cell cycle regulator. It is associated dynamically with chromatin through binding to histones H2A and/or H2B in a Ras-related nuclear (Ran) regulated manner. RCC1 has been shown to play essential roles in nuclear transport, spindle organization and nuclear envelop formation. α-N-methylation of RCC1 is essential for strong attachment of RCC1 to chromatin during normal mitosis.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Ting Chen et al.
Nature cell biology, 9(5), 596-603 (2007-04-17)
Regulator of chromatin condensation 1 (RCC1) is the only known guanine nucleotide-exchange factor for the Ran GTPase and has pivotal roles in nucleo-cytoplasmic transport, mitosis, and nuclear-envelope assembly. RCC1 associates dynamically with chromatin through binding to histones H2A and/or H2B
Ouadah Hadjebi et al.
Biochimica et biophysica acta, 1783(8), 1467-1479 (2008-04-30)
The Regulator of Chromosome Condensation 1 (RCC1) was identified over 20 years ago as a critical cell cycle regulator. By analyzing its amino acid sequence, RCC1 was found to consist of seven homologous repeats of 51-68 amino acid residues, which
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