SAB4200287
抗-肌动蛋白，肌细胞特异性抗体，小鼠单克隆
clone HHF-35, purified from hybridoma cell culture
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
HHF-35, monoclonal
Application:
ICC, IHC, WB
Citations:
4
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
HHF-35, monoclonal
form
buffered aqueous solution
mol wt
antigen ~42 kDa
species reactivity
rat, bovine, canine, chicken, human, mouse, hamster, monkey
concentration
~1.0 mg/mL
technique(s)
immunocytochemistry: 2-4 μg/mL using A10 cells, immunohistochemistry: suitable, western blot: 0.1-0.2 μg/mL using A10 cell extracts
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ACTB(60)
General description
单克隆抗肌动蛋白肌细胞特异性（小鼠IgG1同种型）来源于小鼠骨髓瘤细胞和SDS提取人心肌蛋白免疫的BALB/c小鼠脾细胞融合产生的杂交瘤HHF-35。参与细胞运动的两种主要细胞骨架蛋白是肌动蛋白和肌球蛋白。尽管肌动蛋白是最保守的真核蛋白之一，但可在哺乳动物和禽类中表达为通过电泳迁移率和氨基酸序列分析而得以表征的六种异构体。
Immunogen
SDS提取的人心肌蛋白组分。
Application
该抗体可用于各种免疫化学技术，包括免疫印迹、免疫细胞化学和免疫组织化学。
Biochem/physiol Actions
肌动蛋白和肌球蛋白是许多细胞类型的组成部分，它们参与了无数的细胞过程，包括运动、分泌、细胞质流动、吞噬和细胞分裂。
Physical form
溶于0.01 M磷酸盐缓冲液（pH 7.4）中，含有15 mM叠氮化钠。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的人类或动物食用或应用。
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
常规特殊物品
此项目有
Live cell imaging of the cytoskeleton
Test, 505, 203-217 (2012)
Jose Luis Martin-Ventura et al.
Atherosclerosis, 198(2), 264-271 (2008-04-02)
Parathyroid hormone-related protein (PTHrP) is overexpressed in atherosclerotic plaques by unknown mechanisms. We have examined here the putative mechanism(s) responsible for this overexpression in the atherosclerotic lesion and its potential modulation by simvastatin, both in vitro and in vivo. Atherosclerosis
T Tsukada et al.
The American journal of pathology, 126(1), 51-60 (1987-01-01)
A monoclonal antibody to muscle cell actin isotypes was produced and characterized. Immunocytochemical analysis of methanol-Carnoy's-fixed, paraffin-embedded human tissue revealed that this antibody, termed HHF35, reacts with skeletal muscle cells, cardiac muscle cells, smooth muscle cells, pericytes, and myoepithelial cells
Actin structure and function
Dominguez R, et al.
Annual Review of Biophysics, 40, 169-186 (2011)
相关内容
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