SAB4200319
Anti-PTPRA antibody produced in rabbit
~1.0 mg/mL
别名:
Anti-LRP, Anti-PTPA, Anti-PTPRL2, Anti-PTPase-α, Anti-Tyrosine protein phosphatase receptor type α
登录 查看组织和合同定价。
选择尺寸
关于此项目
NACRES:
NA.44
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, WB
Citations:
4
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~90 kDa
species reactivity
mouse, human
concentration
~1.0 mg/mL
technique(s)
indirect immunofluorescence: 5-10 μg/mL using paraformaldehyde fixed HUVEC cells., western blot: 0.25-0.5 μg/mL using lysates of NIH-3T3 or PC12 cells.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... PTPRA(5786)
mouse ... PTPRA(19262)
General description
PTPRA is a transmembrane phosphatase that regulates cell proliferation, cell adhesion, integrin signaling, and growth factor response. This phosphatase also activates proteins of the Src family. PTPRA inhibits cell growth in breast cancer and contributes to tumorigenesis in colon carcinoma . Anti-PTPRA antibody is specific for human and mouse PTPRA. In immunoblotting, detection of the PTPRA band is specifically inhibited by the immunizing peptide.
Protein tyrosine phosphatase alpha (PTPRA) is a transmembrane phosphatase with a relatively short, highly glycosylated extracellular domain, a transmembrane domain, and a tandem phosphatase domain. The amino-terminal domain predominantly mediates catalytic activity, whereas the carboxyterminal domain serves as a regulatory subunit.
Immunogen
synthetic peptide corresponding to an internal region of human PTPRA, conjugated to KLH. The corresponding sequence is identical in mouse and differs by one amino acid in rat.
Application
Anti-PTPRA antibody produced in rabbit has been used in immunoblotting and immunofluorescence.
Anti-PTPRA antibody is suitable for use in western blot (0.25-0.5 μg/mL using lysates of NIH-3T3 or PC12 cells) and indirect immunofluorescence (5-10 μg/mL using paraformaldehyde fixed HUVEC cells).
Biochem/physiol Actions
Protein tyrosine phosphatase alpha (PTPRA) is implicated in several signaling pathways, including insulin receptor signaling, cellular transformation and cell spreading and migration. It regulates cell proliferation, cell adhesion, integrin signaling, and growth factor response. PTPRA inhibits cell growth in breast cancer and contributes to tumorigenesis in colon carcinoma.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
未找到合适的产品？
试试我们的产品选型工具.
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
K Tabiti et al.
Cancer letters, 93(2), 239-248 (1995-07-13)
The protein tyrosine phosphatase alpha (PTP alpha) mRNA level in paired samples of late stage (Dukes' D) colorectal tumors and adjacent normal colon mucosa was quantified by RNase protection assays. After normalization against 18S RNA or beta-actin mRNA level, a
Receptor-type protein tyrosine phosphatases in cancer
Du Yu and Grandis JR
Chinese Journal of Cancer, 34(2), 61-61 (2015)
N X Krueger et al.
The EMBO journal, 9(10), 3241-3252 (1990-10-01)
Protein tyrosine phosphatases (PTPases), together with protein tyrosine kinases, regulate the tyrosine phosphorylation that controls cell activities and proliferation. Previously, it has been recognized that both cytosolic PTPases and membrane associated, receptor-like PTPases exist. In order to examine the structural
E Ardini et al.
Oncogene, 19(43), 4979-4987 (2000-10-24)
Tyrosine phosphorylation is controlled by a balance of tyrosine kinases (PTKs) and protein tyrosine phosphatases (PTPs). Whereas the contribution of PTKs to breast tumorigenesis is the subject of intense scrutiny, the potential role of PTPs is poorly known. RPTPalpha is
相关内容
Instructions
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持