Anti-OSTF1 (C-terminal) antibody produced in rabbit

Osteoclast-stimulating factor (OSF or OSTF1) gene is mapped to human chromosome 9q21.13 and is expressed in osteoclasts (OCL) as well as motor neurons. OSF is a small intracellular protein and comprises an N-terminal proline-rich region SRC Homology 3 (SH3) domain, three ankyrin repeats and a C-terminal aspartate-rich region. Structurally, OCF protein takes up a β‐barrel fold.

peptide corresponding to the C- terminal region of human OSTF1 conjugated to KLH. The corresponding sequence differs by two or three amino acids in mouse or rat, respectively.

Anti-OSTF1 (C-terminal) antibody produced in rabbit may be used in immunoblotting.

Anti-OSTF1 (C-terminal) recognizes human OSTF1.

Osteoclast-stimulating factor (OSF or OSTF1) SRC Homology 3 (SH3) domain undergoes conformational changes to bind to proline-rich ligand. The C-terminal ankyrin repeats preferably binds to negative-charged binding partners. OSF-SH3 domain interaction stimulates OCL formation and its inhibition may reduce bone resorption by OCL. A compromised bone resorption functionality in OCL further triggers bone loss and may lead to various bone disorders, including hyperparathyroidism, Paget′s disease, osteoporosis, estrogen deficiency, periodontal disease as well as inflammatory bone loss and bone malignancy. OSF favors OCL formation by interacting with sarcoma proto oncogene (c-Src) and its associated proteins based signaling pathway. OSF is implicated in the pathophysiology of spinal muscular atrophy by its possible interaction with survival motor neuron (SMN) proteins through SH3 domain.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

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