SAB4200336
Anti-GUSB (C-terminal) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody
别名:
Anti-β-D-Glucuronidase, Anti-β-Glucuronidase, Anti-β-g1, Anti-MPS7
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IHC, WB
Citations:
3
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 78 kDa
species reactivity
rat, human
concentration
~1.0 mg/mL
technique(s)
immunohistochemistry: 10-20 μg/mL using biotin / ExtrAvidin®-Peroxidase staining of heat-retrieved formalin-fixed, paraffin-embedded rat liver sections, western blot: 2-4 μg/mL using whole extracts of human HepG2 cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... GUSB(2990)
mouse ... Gusb(110006)
General description
Glucuronidase β (GUSB), an exoglycosidase, is a member of the glycosyl hydrolase 2 family. The GUSB protein has a homotetrameric structure that is found in the lysosome. It contains three structural domains, such as a jelly roll barrel, an immunoglobulin constant domain and a triosephosphate isomerase (TIM) barrel respectively. The gene GUSB is mapped to the human chromosome location 7q11.21.
Immunogen
a synthetic peptide corresponding to a sequence at the C-terminal region of human GUSB, conjugated to KLH. The corresponding sequence is identical in mouse and differs by a single amino acid in rat.
Application
Anti-GUSB (C-terminal) antibody produced in rabbit may be used in immunoblotting and immunohistochemistry.
Biochem/physiol Actions
Anti-GUSB (C-terminal) recognizes human and rat GUSB.
Glucuronidase β (GUSB) is a hydrolase that degrades glycosaminoglycans (GAGs), such as heparan sulfate, dermatan sulfate and chondroitin-4,6-sulfate. In the absence of GUS, only partial degradation of GAGs takes place. This leads to the accumulation of GAGs in the lysosomes of many tissues, leading to cellular and organ dysfunction. Mutations in this gene lead to mucopolysaccharidosis type VII (Sly syndrome). Several pseudogenes of this locus are present in the human genome.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8°C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Legal Information
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Mutations and polymorphisms in GUSB gene in mucopolysaccharidosis VII (Sly Syndrome)
Tomatsu S, et al.
Human Mutation, 30(4), 511 -5519 (2009)
The structural basis of substrate recognition in an exo-beta-D-glucosaminidase involved in chitosan hydrolysis
van Bueren AL, et al.
Journal of molecular biology, 385(1), 131 -1139 (2009)
Structure of human beta-glucuronidase reveals candidate lysosomal targeting and active-site motifs
Jain S, et al.
Nature Structural Biology, 3(4), 375 -3381 (1996)
相关内容
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