Anti-XTP3-B antibody, Mouse monoclonal

Monoclonal Anti-XTP3-B (mouse IgG2b isotype) is derived from the hybridoma XTP3-10 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. XTP3-transactivated gene B precursor (XTP3-B) belongs to the mannose-6-phosphate receptor homology (MRH) domain family, which comprises two MRH domains in its sequence. It is a luminal resident protein found in the endoplasmic reticulum. The XTP3-B gene is located at human chromosome 2p16.2.

synthetic peptide corresponding to an internal region of human XTP3-B, conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat.

Monoclonal Anti-XTP3-B antibody produced in mouse may be used in immunoblotting and immunoprecipitation.

Monoclonal Anti-XTP3-B recognizes human XTP3-B.

XTP3-transactivated gene B precursor (XTP3-B) is implicated in endoplasmic reticulum (ER) quality control. It plays a role in ER-associated protein degradation (ERAD) selection and targeting processes. Human XTP3-B is linked with the suppressor of lin-12-like protein 1/ hydroxymethylglutaryl reductase degradation protein 1 (SEL1L/HRD1) ubiquitin ligase complex in the ER membrane and with the ER chaperone binding immunoglobulin protein (BiP) to form an ER quality control scaffold complex. This large complex, which also comprises the osteosarcoma 9 (OS-9) ER lectin, regulates the recognition and sorting of misfolded glycoproteins and non-glycosylated proteins before retrotranslocation.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20oC in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

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