SAB4200353
Anti-acetyl-Histone H4 [Ac-Lys12] antibody produced in rabbit
IgG fraction of antiserum
别名:
Anti-H4, Anti-H4K12ac, Anti-HIST4H4, Anti-Histone cluster 4
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, IHC, WB
Citations:
3
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~12 kDa
species reactivity
mouse, rat, human
technique(s)
immunohistochemistry: 1:100-1:200 using formalin-fixed paraffin-embedded human breast carcinoma, indirect immunofluorescence: 1:250-1:500 using HeLa cells, western blot: 1:500-1:1000 using NIH3T3 cell lysates
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
acetylation (Lys12)
Gene Information
human ... HIST3H3(8290)
General description
Histones H3 and H4 are the predominant histones subjected to extensive covalent modifications. It is an important component of chromatin structure.
Immunogen
synthetic peptide containing acetylated Lys12 of human histone H4, conjugated to KLH. The corresponding sequence is identical in many species including rat and mouse histone H4.
Application
Anti-acetyl-Histone H4 [Ac-Lys12] antibody produced in rabbit has been used in:
- immunoblotting
- immunofluorescence
- immunohistochemistry
Biochem/physiol Actions
Histone H4 can be reversibly acetylated at lysine residues K5, K8, K12 and K16, and the acetylation occurs initially at K16 and then propagates in an N-terminal direction through K12, K8 and K5. Altered acetylation of histone H4 on Lys12 (H4- K12Ac) is linked to age-dependent memory impairment in mice. Histones are subjected to several covalent modifications, such as phosphorylation, methylation, acetylation and ubiquitination, that affect chromatin structure and regulate chromatin activity. Histone modifications plays an important role in cancer and disease.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
此项目有
Altered histone acetylation is associated with age-dependent memory impairment in mice
Peleg S, et al.
Science, 328(5979), 753-756 (2010)
Robert Schneider et al.
Trends in biochemical sciences, 27(8), 396-402 (2002-08-02)
Enzymes that covalently modify histones control many cellular processes by affecting gene expression. A new class of these enzymes is the histone lysine methyltransferase family, whose catalytic activity lies within a conserved domain, the SET domain. This article surveys the
The language of covalent histone modifications
Strahl BD and Allis CD
Nature, 403(6765), 41-41 (2000)
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