SAB4200353
Anti-acetyl-Histone H4 [Ac-Lys12] antibody produced in rabbit
IgG fraction of antiserum
别名:
Anti-H4, Anti-H4K12ac, Anti-HIST4H4, Anti-Histone cluster 4
生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
IgG fraction of antiserum
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen ~12 kDa
种属反应性
mouse, rat, human
技术
immunohistochemistry: 1:100-1:200 using formalin-fixed paraffin-embedded human breast carcinoma
indirect immunofluorescence: 1:250-1:500 using HeLa cells
western blot: 1:500-1:1000 using NIH3T3 cell lysates
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
acetylation (Lys12)
基因信息
human ... HIST3H3(8290)
一般描述
Histones H3 and H4 are the predominant histones subjected to extensive covalent modifications. It is an important component of chromatin structure.
免疫原
synthetic peptide containing acetylated Lys12 of human histone H4, conjugated to KLH. The corresponding sequence is identical in many species including rat and mouse histone H4.
应用
Anti-acetyl-Histone H4 [Ac-Lys12] antibody produced in rabbit has been used in:
- immunoblotting
- immunofluorescence
- immunohistochemistry
生化/生理作用
Histone H4 can be reversibly acetylated at lysine residues K5, K8, K12 and K16, and the acetylation occurs initially at K16 and then propagates in an N-terminal direction through K12, K8 and K5. Altered acetylation of histone H4 on Lys12 (H4- K12Ac) is linked to age-dependent memory impairment in mice. Histones are subjected to several covalent modifications, such as phosphorylation, methylation, acetylation and ubiquitination, that affect chromatin structure and regulate chromatin activity. Histone modifications plays an important role in cancer and disease.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Robert Schneider et al.
Trends in biochemical sciences, 27(8), 396-402 (2002-08-02)
Enzymes that covalently modify histones control many cellular processes by affecting gene expression. A new class of these enzymes is the histone lysine methyltransferase family, whose catalytic activity lies within a conserved domain, the SET domain. This article surveys the
Altered histone acetylation is associated with age-dependent memory impairment in mice
Peleg S, et al.
Science, 328(5979), 753-756 (2010)
The language of covalent histone modifications
Strahl BD and Allis CD
Nature, 403(6765), 41-41 (2000)
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