SAB4200365
Anti-PIWIL1 antibody, Mouse monoclonal
clone PIWIL1-2, purified from hybridoma cell culture
别名:
Monoclonal Anti-PIWIL1 antibody produced in mouse, Monoclonal Anti-HIWI, Monoclonal Anti-MIWI, Monoclonal Anti-PIWI, Monoclonal Anti-piwi-like 1
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
PIWIL1-2, monoclonal
Application:
IHC, IP, WB
Citations:
8
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
PIWIL1-2, monoclonal
form
buffered aqueous solution
mol wt
antigen ~95 kDa
species reactivity
human, mouse
concentration
~1.0 mg/mL
technique(s)
immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded mouse testis and biotin / ExtrAvidin® Peroxidase staining system., immunoprecipitation (IP): suitable, western blot: 2.5-5.0 μg/mL using whole cell extracts of HEK-293T cells over-expressing PIWIL1.
isotype
IgG2b
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... PIWIL1(9271)
mouse ... Piwil1(57749)
General description
Monoclonal Anti-PIWIL1 (mouse IgG2b isotype) is derived from the hybridoma piwi-like protein 1 (PIWIL1-2) produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to an internal region of human PIWIL1 conjugated to KLH. PIWIL1 is a member of Piwi subfamily. It is expressed mostly to the germline. The encoded protein consists of the PAZ domain and the PIWI domain. The PIWIL1 gene is located on human chromosome 12q24.33.
Immunogen
synthetic peptide corresponding to an internal region of human PIWIL1, conjugated to KLH. The corresponding sequence differs by one amino acid in mouse PIWIL1.
Application
Monoclonal Anti-PIWIL1 antibody produced in mouse has been used in:
- immunofluorescence
- immunoprecipitation
- flow cytometry analysis29}
- immunoblotting
- immunohistochemistry
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)
Immunoprecipitation (1 paper)
Biochem/physiol Actions
Piwi proteins are implicated in epigenetic control of gene expression, transposon silencing and translation regulation. It plays vital roles in the germline development and gametogenesis of many metazoan species.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Legal Information
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
此项目有
The biogenesis and function of PIWI proteins and piRNAs: progress and prospect
Thomson T and Lin H
Annual Review of Cell and Developmental Biology, 25, 355-376 (2009)
Molecular characterization of hiwi, a human member of the piwi gene family whose overexpression is correlated to seminomas
Qiao D, et al.
Oncogene, 21(25), 3988-3988 (2002)
An Lnc RNA (GAS5)/SnoRNA-derived piRNA induces activation of TRAIL gene by site-specifically recruiting MLL/COMPASS-like complexes
He X, et al.
Nucleic Acids Research, 43(7), 3712-3725 (2015)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| SAB4200365-200UL | 04061837795466 |