Anti-acetyl-Histone H3 [Ac-Lys²³] antibody produced in rabbit

Histone H3 is a component of the histone octamer of the nucleosome DNA complex. It possesses a lysine-rich N-terminal tail region. Histone H3 gene is mapped to human chromosome 1q42.13.

synthetic acetylated peptide containing acetylated Lys23 of human histone H3, conjugated to KLH. The corresponding sequence is identical in many species including rat and mouse histone H3.

Anti-acetyl-Histone H3 [Ac-Lys²³] antibody produced in rabbit may be used in:

• immunoblotting
• immunofluorescence
• immunohistochemistry

Anti-Acetyl-Histone H3 [Ac-Lys23] specifically recognizes human and mouse [Ac-Lys23] Histone H3.

Histones H3 and H4 are the predominant histones subjected to extensive covalent modifications. Active chromatin is also correlated with the hyperacetylation of histone tail. Histone H3 can be reversibly acetylated at Lys residues 9, 14, 18, 23, 27, 36 and 56. Histone H3 is acetylated at Lys18 by E1A binding protein p300/CREB-binding protein (CBP/p300) following estrogen stimulation, leading to acetylation of Lys23, and methylation of Arg17 by coactivator associated arginine methyltransferase 1 (CARM1). These events lead to transcriptional activation of the estrogen-responsive genes. Renal failure has been shown to increase cardiac histone H3 acetylation of Lys23 and Lys9, dimethylation of Lys4. Phosphorylation of Ser10 along with lysine dimethylation is reported to induce cardiomyopathy-related genes in a mice model of type 2 diabetes.

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

For continuous use, store at 2-8 °C for upto one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

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