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Merck
CN

SAB4200392

Sigma-Aldrich

Anti-BRSK1 antibody produced in rabbit

enhanced validation

~1.5 mg/mL, affinity isolated antibody

别名:

Anti-BR serine-threonine kinase 1, Anti-SAD-B, Anti-SAD1 kinase

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关于此项目

UNSPSC代码:
12352203
NACRES:
NA.44
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生物来源

rabbit

偶联物

unconjugated

抗体形式

affinity isolated antibody

抗体产品类型

primary antibodies

克隆

polyclonal

表单

buffered aqueous solution

分子量

antigen ~90 kDa

种属反应性

mouse

增强验证

recombinant expression
Learn more about Antibody Enhanced Validation

浓度

~1.5 mg/mL

技术

immunoprecipitation (IP): 10-20 μg using lysates of HEK-293T cells overexpressing mouse BRSK1
indirect immunofluorescence: 0.2-0.4 μg/mL using HEK-293T cells overexpressing mouse BRSK1
western blot: 2-4 μg/mL using extracts of mouse forebrain (S1 fraction)

UniProt登记号

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

基因信息

human ... BRSK1(84446)
mouse ... Brsk1(381979)

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一般描述

BRSK1 (BR serine/threonine kinase 1), also known as serine/threonine-protein kinase (SAD-1, SAD-B) and BRSK2 (SAD-A) belong to the family of Ser/Thr AMP-activated protein kinase (AMPK)-related kinases that are specifically expressed in the mammalian brain. BRSK1 gene is mapped to human chromosome 19q13.42. BRSK1 protein localizes in the synaptic vesicle in the hippocampus and cerebellum.

免疫原

synthetic peptide corresponding to an internal sequence of human BRSK1, conjugated to KLH. The corresponding sequence is identical in mouse BRSK1.

应用

Anti-BRSK1 antibody is suitable for use in western blot (2-4 μg/mL using S1 fraction extracts of mouse forebrain). The product can also be used for immunoprecipitation (10-20 μg) and indirect immunofluorescence (0.2-0.4 μg/mL) using HEK-293T cells overexpressing mouse BRSK1.

生化/生理作用

Anti-BRSK1 specifically recognizes mouse BRSK1.
BRSK1 (BR serine/threonine kinase 1) is essential for the polarization of cortical neurons. Knock-out mice that lack both BRSK1 and BRSK2 have defects in neuronal polarity and die prematurely after birth. Liver kinase B1(LKB1) phosphorylates and activates the serine/threonine-protein (SAD) kinases at a specific threonine residue within the T-loop activation segment of the kinase domain. BRSK1/2 in turn phosphorylates downstream effectors such as the microtubule-associated protein, tau and the cell cycle checkpoint kinase, Wee1-like protein kinase (Wee1). Phosphorylation of Wee1 by BRSK1/2 is required to regulate its activity and differentiation of polarized neurons. Also, BRSK1/SADB is localized to centrosomes and to control centrosome duplication. SADB phosphorylates γ-tubulin on Ser131 residue, suggesting that it controls centrosome homeostasis by regulating the phosphorylation of γ -tubulin.
BRSK1 is a serine/threonine kinase that facilitates the G(2)/M cell cycle arrest upon UV- or methyl methane sulfonate-induced DNA damage. This kinase also modulates the polarity of neurons and duplication of centrosomes. Anti-BRSK1 antibody is specific for mouse BRSK1. The antibody is also expected to react with human BRSK1. In immunoblotting, detection of the BRSK1 band is specifically inhibited by the BRSK1 immunizing peptide.

外形

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

制备说明

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

10 - Combustible liquids

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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Genetics of reproductive lifespan.
Patricia Hartge
Nature genetics, 41(6), 637-638 (2009-05-28)
Arantza Rodríguez-Asiain et al.
Biochimica et biophysica acta, 1811(12), 1124-1135 (2011-10-25)
Brain specific kinases 1 and 2 (BRSK1/2, also named SAD kinases) are serine-threonine kinases specifically expressed in the brain, and activated by LKB1-mediated phosphorylation of a threonine residue at their T-loop (Thr189/174 in human BRSK1/2). BRSKs are crucial for establishing
Rui Lu et al.
The Journal of biological chemistry, 279(30), 31164-31170 (2004-05-20)
Checkpoint activation by DNA damage during G(2) prevents activation of cyclin B/Cdc2 complexes, and as a consequence, mitotic entry is blocked. Although initiation and maintenance of G(2) arrest are known to be regulated by at least two distinct signaling pathways
María Alvarado-Kristensson et al.
Nature cell biology, 11(9), 1081-1092 (2009-08-04)
Symmetrical cell division requires duplication of DNA and protein content to generate two daughter cells. Centrosomes also duplicate during cell division, but the mechanism controlling this process is incompletely understood. We describe an alternative splice form of SadB encoding a
Masashi Kishi et al.
Science (New York, N.Y.), 307(5711), 929-932 (2005-02-12)
Electrical activity in neurons is generally initiated in dendritic processes then propagated along axons to synapses, where it is passed to other neurons. Major structural features of neurons-their dendrites and axons-are thus related to their fundamental functions: the receipt and

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