SAB4200393
Anti-BRSK1 (C-terminal) antibody produced in rabbit
~1.5 mg/mL, affinity isolated antibody
别名:
Anti-BR SERINE/THREONINE KINASE 1, Anti-SAD-B
登录 查看组织和合同定价。
选择尺寸
关于此项目
NACRES:
NA.44
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, IP, WB
Citations:
3
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~90 kDa
species reactivity
human, mouse
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
concentration
~1.5 mg/mL
technique(s)
immunoprecipitation (IP): 10-20 μg using HEK-293T cell lysates overexpressing mouse BRSK1., indirect immunofluorescence: 0.2-0.4 μg/mL using HEK-293T cells overexpressing mouse BRSK1., western blot: 1-2 μg/mL using mouse forebrain (S1 fraction).
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... BRSK1(84446)
mouse ... Brsk1(381979)
General description
BRSK1 is a serine/threonine kinase that facilitates the G(2)/M cell cycle arrest upon UV- or methyl methane sulfonate-induced DNA damage. This kinase also modulates the polarity of neurons and duplication of centrosomes . Anti-BRSK1 (C-terminal) antibody is specific for mouse and human BRSK1. In immunoblotting, detection of the BRSK1 band is specifically inhibited by the BRSK1 immunizing peptide.
Immunogen
synthetic peptide corresponding to a sequence at the C-terminal of human BRSK1, conjugated to KLH. The corresponding sequence is identical in mouse BRSK1.
Application
Anti-BRSK1 (C-terminal) antibody is suitable for use in western blot (1-2 μg/mL using S1 fraction extracts of mouse forebrain). The product can also be used for immunoprecipitation (10-20 μg) and indirect immunofluorescence (0.2-0.4 μg/mL) using HEK-293T cells overexpressing mouse BRSK1.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
未找到合适的产品？
试试我们的产品选型工具.
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Rui Lu et al.
The Journal of biological chemistry, 279(30), 31164-31170 (2004-05-20)
Checkpoint activation by DNA damage during G(2) prevents activation of cyclin B/Cdc2 complexes, and as a consequence, mitotic entry is blocked. Although initiation and maintenance of G(2) arrest are known to be regulated by at least two distinct signaling pathways
María Alvarado-Kristensson et al.
Nature cell biology, 11(9), 1081-1092 (2009-08-04)
Symmetrical cell division requires duplication of DNA and protein content to generate two daughter cells. Centrosomes also duplicate during cell division, but the mechanism controlling this process is incompletely understood. We describe an alternative splice form of SadB encoding a
Myriam Müller et al.
Journal of cell science, 123(Pt 2), 286-294 (2009-12-23)
Wee1 is well characterized as a cell-cycle checkpoint kinase that regulates the entry into mitosis in dividing cells. Here we identify a novel function of Wee1 in postmitotic neurons during the establishment of distinct axonal and dendritic compartments, which is
相关内容
Instructions
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持