Anti-DIS3 antibody, Mouse monoclonal

Monoclonal Anti-DIS3 (mouse IgG1 isotype) is derived from the hybridoma DIS3-3 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the C-terminal region of human DIS3, conjugated to keyhole limpet hemocyanin (KLH). DIS3 homolog, exosome endoribonuclease and 3′-5′ exoribonuclease (DIS3) mitotic control homolog is a catalytic component of the eukaryotic RNA exosome. The RNA exosome is a multi-subunit complex with ribonucleolytic complex of 400 kDa and nine subunit which forms the catalytically inactive core. The exosome complex is localized to the nucleus and the cytoplasm. DIS3 is mainly nuclear, whereas DIS3L is confined to cytoplasm.

synthetic peptide corresponding to a sequence at the C-terminal region of human DIS3, conjugated to KLH.

Monoclonal Anti-DIS3 antibody produced in mouse has been used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

DIS3 homolog, exosome endoribonuclease and 3′-5′ exoribonuclease (DIS3) is involved in RNA processing and turnover. Its core subunit maintains structural function and is involved in substrate recognition. The exosome is important for the 3′ → 5′ processing and degradation of many types of RNAs including mRNA, rRNA, snRNA, snoRNA and tRNA. DIS3 and DIS3L are active exonucleases. DIS3 also displays endonuclease activity.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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