SAB4200507
Anti-ATG101 antibody, Mouse monoclonal
clone ATG101-7, purified from hybridoma cell culture
别名:
Anti-C12orf44, chromosome 12 open reading frame 44, PP894, Atg13-interacting protein, Anti-autophagy-related protein 101, Monoclonal Anti-ATG101 antibody produced in mouse
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
ATG101-7, monoclonal
Application:
WB
Citations:
4
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
ATG101-7, monoclonal
form
buffered aqueous solution
mol wt
antigen ~30 kDa
species reactivity
human
concentration
~1.0 mg/mL
technique(s)
western blot: 1-2 μg/mL using whole extracts of HEK-293T cells overexpressing human ATG101.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... C12orf44(60673)
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General description
Monoclonal Anti-ATG101 (mouse IgG1 isotype) is derived from the hybridoma ATG101-7 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Atg101 is conserved in humans and several eukaryotes. It is not conserved in yeast Saccharomyces cerevisiae. ATG101 gene is located on the human chromosome at 12q13.13.
Immunogen
synthetic peptide corresponding to the N-terminal region of human ATG101, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey, canine and bovine ATG101.
Application
Monoclonal Anti-ATG101 antibody produced in mouse is suitable for use in immunoblotting and immunoprecipitation.
Biochem/physiol Actions
Anti- ATG101 recognizes human ATG101.
Atg101 is a mammalian Atg13 binding protein essential for autophagagy. Atg13 forms a stable complex with Unc-51 like autophagy activating kinase 1 (ULK1) and focal adhesion kinase (FAK) family kinase-interacting protein of 200 kDa (FIP200). Atg101 is linked to the ULK1-Atg13-FIP200 complex through direct contact with Atg13.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20°C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Investigation of Androgen Responsive Elements in Some Autophagy Related Genes via In Silico Analysis.
Erzurumlu Y
Journal of Health Science, 11(1) (2020)
A novel, human Atg13 binding protein, Atg101, interacts with ULK1 and is essential for macroautophagy
Mercer C A, et al.
Autophagy, 5(5), 649-662 (2009)
Atg101, a novel mammalian autophagy protein interacting with Atg13
Hosokawa N, et al.
Autophagy, 5(7), 973-979 (2009)
Structure of the human Atg13-Atg101 HORMA heterodimer: an interaction hub within the ULK1 complex
Qi S, et al.
Structure, 23(10), 1848-1857 (2015)
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