SAB4200533
Anti-Villin-1 (internal) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody
别名:
Anti-D2S1471, Anti-VIL
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, IHC
Citations:
4
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~95 kDa
species reactivity
human
concentration
~1.0 mg/mL
technique(s)
immunoblotting: 1-2 μg/mL using extracts of WiDR cells, immunofluorescence: 5-10 μg/mL using Caco-2 cells, immunohistochemistry: 20 μg/mL using human small intestine
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... VIL1(7429)
General description
Villin is also known as villin-1 or VIL1 is a Ca2+ -regulated actin-binding protein. It belongs to the gelsolin family. Villin is localized in the apical cytoplasm and brush borders of epithelial cells of the small and large intestinal mucosa, intrahepatic bile ducts, kidney proximal tubules, gall bladder epithelium and pancreatic ducts. It is located on the human chromosome at 2q35.
Immunogen
synthetic peptide corresponding to an internal sequence of human villin-1, conjugated to KLH. The corresponding sequence has high homology (81% identity) to mouse villin-1.
Application
Anti-Villin-1 (internal) antibody produced in rabbit may be used in:
- immunoblotting
- immunofluorescence
- immunohistochemistry
Biochem/physiol Actions
Anti-Villin-1 (internal) specifically recognizes human villin-1.
Villin functions in modulating the structure and assembly of actin filaments. It also plays a role in modulating cell migration by phosphorylating villin at Tyr-60, Tyr-81 and Tyr-256.Villin plays a role in microvillus assembly and acts as a marker for epithelial cell differentiation. Expression of villin is stimulated during intestinal metaplasia observed in Barrett′s esophagus and chronic atrophic gastritis. Expression of villin is increased during human adenocarcinomas, such as colorectal cancer.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Dynamic effects of acid on Barrett's esophagus. An ex vivo proliferation and differentiation model.
Fitzgerald R C, et al.
The Journal of Clinical Investigation, 98(9), 2120-2128 (1996)
Villin expression is frequently lost in poorly differentiated colon cancer
Arango D, et al.
The American Journal of Pathology, 180(4), 1509-1521 (2012)
Dendritic spine actin cytoskeleton in autism spectrum disorder
Joensuu M, et al.
Progress in Neuro-Psychopharmacology & Biological Psychiatry, 84(14), 362-381 (2018)
Seema Khurana et al.
FEBS letters, 582(14), 2128-2139 (2008-03-01)
Villin is a tissue-specific actin modifying protein that is associated with actin filaments in the microvilli and terminal web of epithelial cells. It belongs to a large family of actin-binding proteins which includes actin-capping, -nucleating and/or -severing proteins such as
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