生物来源
rabbit
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen ~39 kDa
种属反应性
human
浓度
~1.0 mg/mL
技术
immunoblotting: 1-2 μg/mL using Lysates of HEK-293T cells overexpressing human Msi-1.
immunoprecipitation (IP): 5-10 μg using HEK-293T cells overexpressing human Msi-1.
indirect immunofluorescence: 5-10 μg/mL using HeLa cells.
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... MSI1(4440)
rat ... Msi1(259272)
一般描述
Musashi-1 (Msi-1) is highly expressed in neural stem/progenitor cells (NS/PCs). MSI1 gene is mapped to human chromosome 12q24.
免疫原
synthetic peptide corresponding to an internal sequence of human Msi-1, conjugated to KLH. The corresponding sequence is identical in rat and highly conserved (94% identity) in mouse Msi-1.
应用
Anti-Msi-1 antibody produced in rabbit has been used in:
- immunoblotting
- immunoprecipitation
- immunofluorescence
生化/生理作用
Musashi-1 (Msi-1) is a neural RNA-binding protein that regulate translation of target mRNAs. It is associated with maintenance and asymmetric cell division of neural stem cells. Msi-1 binds to the 3′-untranslated region (UTR) of its target mRNAs in neural stem/progenitor cells (NS/PCs), repressing their translation, and interfering with NS/PC differentiation thus maintaining NP/PCs in the undifferentiated state. Loss of Msi-1 function disrupts the balance between germ-line stem cell renewal and differentiation, causing premature differentiation. Msi-1 has been shown to regulate the Notch and Wnt signaling pathway. Upregulation of Msi-1 augments Notch signaling, via translational repression of the Notch signaling antagonist m-Numb, thereby inhibiting the differentiation of neural stem cells into neurons. Msi-1 is also expressed in the nucleus during the early neural differentiation of mouse embryonic stem cells (ESCs) and found to act synergistically with Lin28 as a novel cofactor for the blockade of let-7 family miRNA biogenesis. Expression of Msi1- has been detected in several human cancers including brain tumors, suggesting its role in oncogenic development.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
此项目有
Musashi1 cooperates in abnormal cell lineage protein 28 (Lin28)-mediated let-7 family microRNA biogenesis in early neural differentiation
Kawahara H, et al.
The Journal of Biological Chemistry, 286(18), 16121-16130 (2011)
Musashi1: an evolutionally conserved marker for CNS progenitor cells including neural stem cells
Kaneko Y, et al.
Developmental Neuroscience, 22(1-2), 139-139 (2000)
The neural RNA-binding protein Musashi1 translationally regulates mammalian numb gene expression by interacting with its mRNA
Imai T, et al.
Molecular and Cellular Biology, 21(12), 3888-3900 (2001)
RNA-binding protein Musashi family: roles for CNS stem cells and a subpopulation of ependymal cells revealed by targeted disruption and antisense ablation
Sakakibara S, et al.
Proceedings of the National Academy of Sciences of the USA, 99(23), 15194-15199 (2002)
The RNA-binding protein Musashi1 affects medulloblastoma growth via a network of cancer-related genes and is an indicator of poor prognosis
Vo DT, et al.
The American Journal of Pathology, 181(5), 1762-1772 (2012)
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