跳转至内容
Merck
CN

SAB4200586

Anti-POLR2A antibody, Mouse monoclonal

clone RPOL-1, purified from hybridoma cell culture

别名:

Monoclonal Anti-POLR2A antibody produced in mouse, Monoclonal Anti220kDa, Monoclonal AntiPOLR2, Monoclonal AntiPOLRA, Monoclonal AntiRPB1, Monoclonal AntiRPBh1, Monoclonal AntiRPO2, Monoclonal AntiRPOL2, Monoclonal AntiRpIILS, Monoclonal AntihRPB220, Monoclonal AntihsRPB1, Monoclonal Antipolymerase (RNA) II (DNA directed) polypeptide A

登录 查看组织和合同定价。

选择尺寸

关于此项目

NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
RPOL-1, monoclonal
Application:
—
Citations:
4

主要文件

查看所有文档
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

RPOL-1, monoclonal

form

buffered aqueous solution

mol wt

antigen ~250 kDa

species reactivity

human

concentration

~1 mg/mL

technique(s)

immunoblotting: 1-2 μg/mL using nuclear extracts of human HeLa cells.

isotype

IgG1

UniProt accession no.

P24928

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... POLR2A(5430)
mouse ... Polr2a(20020)
rat ... Polr2a(363633)

General description

Monoclonal Anti-POLR2A (mouse IgG1 isotype) is derived from the hybridoma RPOL-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Polymerase (RNA) II (DNA directed) polypeptide A (POLR2A) is the largest subunit of RNA polymerase II. It is mapped on the human chromosome at 17p13.1.

Immunogen

Synthetic peptide corresponding to the N-terminal region of human POLR2A, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey, bovine, dog and pig.

Application

Monoclonal Anti-POLR2A antibody produced in mouse may be used in immunoblotting.

Biochem/physiol Actions

Monoclonal Anti-POLR2A recognizes human POLR2A.
Polymerase (RNA) II (DNA directed) polypeptide A (POLR2A) plays a role in the synthesis of messenger RNA in eukaryotes. It contains a carboxy-terminal domain composed of heptapeptide repeats that are essential for polymerase activity. POLR2A in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20°C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

未找到合适的产品？  

试试我们的产品选型工具.

存储类别

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

新产品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
033M4757V
033N4757V
033M4757

没有发现合适的版本？

如果您需要特殊版本，可通过批号或批次号查找具体证书。

搜索COA

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

RNA polymerase II holoenzyme and transcriptional regulation
Greenblatt, Jack
Current Opinion in Cell Biology, 9(3), 310-319 (1997)
SURVEY AND SUMMARY: Spatial organization of RNA polymerase II transcription in the nucleus
Szentirmay M N and Sawadogo M
Nucleic Acids Research, 28(10), 2019-2025 (2000)
Pre-mRNA processing and the CTD of RNA polymerase II: the tail that wags the dog?
Steinmetz E J
Cell, 89(4), 491-494 (1997)
HDP101, a novel B-cell maturation antigen (BCMA)-targeted antibody conjugated to alpha-Amanitin, is active against myeloma with preferential efficacy against pre-clinical models of deletion 17p
Singh R K, et al.
Blood, 132(Supplement 1), 593-593 (2018)

相关内容

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持