Anti-NSF (C-terminal) antibody produced in rabbit

N-ethylmaleimide-sensitive fusion protein (NSF) is a homotrimer and is made up of three domains such as N-terminal domain and two homologous adenosine triphosphate (ATP)-binding domains. It is located on the human chromosome at 17q21.31.

synthetic peptide corresponding to a sequence located in the C-terminus of human NSF, conjugated to KLH. The corresponding sequence is highly conserved (single amino acid substitution) in rat and mouse NSF.

Anti-NSF (C-terminal) antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunocytochemistry
• immunohistochemistry
• immunofluorescence

N-ethylmaleimide-sensitive fusion protein (NSF) is an essential component of the protein machinery responsible for various membrane fusion events, including inter-cisternal Golgi protein transport and the exocytosis of vesicles. NSF takes part in NSF-dependent membrane fusion in which interaction of two types of cytosolic proteins, NSF and α-, β- and γ- soluble NSF attachment protein (SNAP) isoforms, with the subcellular compartment-specific SNAP receptors (SNAREs) of the vesicle and target membranes. NSF is required in long-term potentiation (LTP), underlying the formation of long-term memory, by regulating the exocytosis of glutamate α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) glutamate receptor 2 (GluR2) at postsynaptic densities (PSD).

Anti-NSF(C-terminal) specifically recognizes human, rat and mouse NSF.

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

For continuous use,store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

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