SAB4200605
Anti-EB3 (C-terminal) antibody produced in rabbit
IgG fraction of antiserum
别名:
Anti-EBF3, Anti-EBF3-S, Anti-MAPRE3, Anti-RP/EB family, Anti-RP3, Anti-member 3, Anti-microtubule-associated protein
生物来源
rabbit
偶联物
unconjugated
抗体形式
IgG fraction of antiserum
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen ~32 kDa
种属反应性
rat, human, mouse
技术
immunoblotting: 1:2,500-1:5,000 using lysates of mouse brain (S1 fraction).
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... EBF3(253738)
一般描述
End-binding protein-3 (EB3), also known as microtubule (MT)-associated protein RP/EB family member 3 (MAPRE3), belongs to a distinct family of “plus-end tracking proteins” (+TIPs). In humans, EB1 family proteins consist of EB1, EB2 and EB3. EB proteins are components of the MT cytoskeleton and are greatly conserved. EB proteins contain an N-terminal calponin homology (CH) domain and a unique EB-like C-terminal motif. EB3 is preferentially expressed in the central nervous system (CNS) and binds to a CNS-specific adenomatous polyposis coli (APC) homolog APCL.
免疫原
synthetic peptide corresponding to a sequence at the C-terminus of human EB3, conjugated to KLH. The corresponding sequence is identical in rat and mouse EB3.
应用
Anti-EB3 (C-terminal) antibody produced in rabbit has been used in:
- immunoblotting
- immunofluorescence
- immunohistochemistry
生化/生理作用
End-binding protein-3 (EB3) plays an important role in regulating dynamics and organization of microtubules (MTs). EB3 is specifically expressed during myogenic differentiation and controls MTs dynamics at the cell cortex. Knockout of EB3 prevents myotubes formation by inhibiting myoblasts elongation and fusion. In neurons, EB3 is found to interact with scaffold protein ankyrin-G and postsynaptic density protein 95 (PSD95) and is required for stabilizing neuronal polarity in mature neurons and in determining neuronal dendritic morphology. EB3 is also involved in MT polymerization and cell migration. EB3 may be involved in stabilizing MTs and anchoring them to centrosomes during spindle formation.
外形
solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
此项目有
Visualization of microtubule growth in cultured neurons via the use of EB3-GFP (end-binding protein 3-green fluorescent protein)
Stepanova T, et al.
The Journal of Neuroscience, 23(7), 2655-2664 (2003)
EB1 and EB3 promote cilia biogenesis by several centrosome-related mechanisms
Schroder JM, et al.
Journal of Cell Science, 124(15), 2539-2551 (2011)
EB3, a novel member of the EB1 family preferentially expressed in the central nervous system, binds to a CNS-specific APC homologue
Nakagawa H, et al.
Oncogene, 19(2), 210-210 (2000)
Characterization of functional domains of human EB1 family proteins
Bu W and Su LK
The Journal of biological chemistry, 278(50), 49721-49731 (2003)
EB3 regulates microtubule dynamics at the cell cortex and is required for myoblast elongation and fusion
Straube and Merdes A
Current Biology, 17(15), 1318-1325 (2007)
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