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Merck
CN

SAB4200605

Sigma-Aldrich

Anti-EB3 (C-terminal) antibody produced in rabbit

IgG fraction of antiserum

别名:

Anti-EBF3, Anti-EBF3-S, Anti-MAPRE3, Anti-RP/EB family, Anti-RP3, Anti-member 3, Anti-microtubule-associated protein

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关于此项目

UNSPSC代码:
12352203
NACRES:
NA.41
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生物来源

rabbit

偶联物

unconjugated

抗体形式

IgG fraction of antiserum

抗体产品类型

primary antibodies

克隆

polyclonal

表单

buffered aqueous solution

分子量

antigen ~32 kDa

种属反应性

rat, human, mouse

技术

immunoblotting: 1:2,500-1:5,000 using lysates of mouse brain (S1 fraction).

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

基因信息

human ... EBF3(253738)

一般描述

End-binding protein-3 (EB3), also known as microtubule (MT)-associated protein RP/EB family member 3 (MAPRE3), belongs to a distinct family of “plus-end tracking proteins” (+TIPs). In humans, EB1 family proteins consist of EB1, EB2 and EB3. EB proteins are components of the MT cytoskeleton and are greatly conserved. EB proteins contain an N-terminal calponin homology (CH) domain and a unique EB-like C-terminal motif. EB3 is preferentially expressed in the central nervous system (CNS) and binds to a CNS-specific adenomatous polyposis coli (APC) homolog APCL.

免疫原

synthetic peptide corresponding to a sequence at the C-terminus of human EB3, conjugated to KLH. The corresponding sequence is identical in rat and mouse EB3.

应用

Anti-EB3 (C-terminal) antibody produced in rabbit has been used in:
  • immunoblotting
  • immunofluorescence
  • immunohistochemistry

生化/生理作用

End-binding protein-3 (EB3) plays an important role in regulating dynamics and organization of microtubules (MTs). EB3 is specifically expressed during myogenic differentiation and controls MTs dynamics at the cell cortex. Knockout of EB3 prevents myotubes formation by inhibiting myoblasts elongation and fusion. In neurons, EB3 is found to interact with scaffold protein ankyrin-G and postsynaptic density protein 95 (PSD95) and is required for stabilizing neuronal polarity in mature neurons and in determining neuronal dendritic morphology. EB3 is also involved in MT polymerization and cell migration. EB3 may be involved in stabilizing MTs and anchoring them to centrosomes during spindle formation.

外形

solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

10 - Combustible liquids

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

新产品
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分析证书(COA)

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Visualization of microtubule growth in cultured neurons via the use of EB3-GFP (end-binding protein 3-green fluorescent protein)
Stepanova T, et al.
The Journal of Neuroscience, 23(7), 2655-2664 (2003)
EB1 and EB3 promote cilia biogenesis by several centrosome-related mechanisms
Schroder JM, et al.
Journal of Cell Science, 124(15), 2539-2551 (2011)
EB3, a novel member of the EB1 family preferentially expressed in the central nervous system, binds to a CNS-specific APC homologue
Nakagawa H, et al.
Oncogene, 19(2), 210-210 (2000)
Characterization of functional domains of human EB1 family proteins
Bu W and Su LK
The Journal of biological chemistry, 278(50), 49721-49731 (2003)
EB3 regulates microtubule dynamics at the cell cortex and is required for myoblast elongation and fusion
Straube and Merdes A
Current Biology, 17(15), 1318-1325 (2007)

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