Monoclonal Anti-EPS8 antibody produced in mouse

Monoclonal Anti- EPS8 (mouse IgG2a isotype) is derived from the hybridoma EPS8-2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the N-terminal region of human EPS8 (GeneID: 2059), conjugated to Key loop hemocyanin (KLH). Epidermal growth factor receptor kinase substrate 8 (EPS8) is also known as DFNB102 (autosomal recessive deafness-102). EPS8 is also found to be an actin filament barbed end capping protein.

synthetic peptide corresponding to a sequence at the N-terminal region of human EPS8

Monoclonal Anti-EPS8 antibody produced in mouse has been used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence
• flow cytometry

Epidermal growth factor receptor kinase substrate 8 (EPS8) serves as a substrate for the kinase activity of epidermal growth factor receptor (EGFR). It is essential for cell motility and regulation of actin filaments growth. EPS8 scaffolds together with Abl interactor 1 (Abi1) and SOS Ras/Rac guanine nucleotide exchange factor 1 (Sos1) to a trimeric complex which is required for activation of the small guanosine triphosphatase (GTPase) Rac. On the other hand, EPS8 form complexes with related to the N terminus of tre oncogene (RN-tre), a Rab5 GTPase activating protein, to inhibit EGFR internalization. EPS8 is involved in the regulation of processes such as axonal filopodia growth, stereocilia length, dendritic cell migration, cancer cell migration and invasion. It is reported to be expressed at elevated levels in a range of human malignancies. This protein is also involved in spine morphogenesis and plasticity and may characterize forms of intellectual disabilities. For instance, reduced EPS8 levels were reported in brains of autism patients.

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

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