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SAB4200684

Sigma-Aldrich

Monoclonal Anti-Uvomorulin/E-Cadherin antibody produced in rat

clone DECMA-1, purified from hybridoma cell culture

别名:
LCAM, CDHE, cadherin 1, type 1, CD324, E-cadherin (epithelial), Arc-1, ECAD, UVO
NACRES:
NA.41

质量水平

200

生物来源

rat

偶联物

unconjugated

抗体形式

purified immunoglobulin

antibody product type

primary antibodies

克隆

DECMA-1, monoclonal

形式

buffered aqueous solution

分子量

antigen ~120 kDa

species reactivity

dog, mouse

浓度

~1 mg/mL

technique(s)

immunoblotting: 0.5-1 μg/mL using whole extract of MDCK cells.
immunofluorescence: 5-10 μg/mL using MDCK cells
immunoprecipitation (IP): suitable

同位素/亚型

IgG1

运输

dry ice

储存温度

−20°C

一般描述

Monoclonal Anti-Uvomorulin/E-Cadherin (rat IgG1 isotype) is derived from the DECMA-1 hybridoma produced by the fusion of rat myeloma cells and splenocytes from an immunized Lou rat. Uvomorulin/E-Cadherin (Epithelial cadherin), also known as CDH1, CAM 120/80, L-CAM (Cell-Adhesion Molecule) or CD324, is a member of the cadherin transmembrane glycoproteins superfamily. It is characterized with a highly conserved cytoplasmic tail.

免疫原

Mouse embryonal carcinoma cell line PCC4 Aza RI

应用

Monoclonal Anti-Uvomorulin/E-Cadherin antibody produced in rat has been used in various immunochemical techniques including immunoblotting, immunoprecipitation, immunohistochemical staining and immunofluorescent staining.

生化/生理作用

E-cadherin is a calcium-dependent glycoprotein. It is responsible for the homogeneous attachment between cells. It suppresses malignant transformation of tumors. Additionally, it also regulates cellular invasion and metastasis.
Cadherins contribute to epithelial cell behavior and tissue morphogenesis/remodeling by facilitating cell-cell adhesion. Monoclonal Anti-Uvomorulin/E-Cadherin can be used for studies of embryonal development, cell-cell interaction of cells grown in culture, and localization of E-Cadherin. In addition, it is commonly used as an E-cadherin inhibitor/blocking antibody in a study of embryonic stem cells (EMS).

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

12 - Non Combustible Liquids

闪点(F)

Not applicable

闪点(C)

Not applicable

分析证书

原产地证书 (CofO)

Cadherin-mediated cell-cell interactions in normal and cancer cells
Gloushankova NA, et al.
Tissue barriers, 5(3), e1356900-e1356900 (2017)
Repetto O., et al.,
BioMed Research International, 2014, 408047-408047 (2014)
Adherens and tight junctions: structure, function and connections to the actin cytoskeleton
Hartsock A and Nelson WJ
Biochimica et Biophysica Acta - Biomembranes, 1778(3), 660-669 (2008)
Yan Zhang et al.
Molecular medicine reports, 12(3), 3393-3399 (2015-05-16)
The aim of the present study was to investigate the expression and clinical significance of hypoxia-inducible factor 1α (HIF-1α), Snail and E-cadherin in ovarian cancer. The expression levels were assessed in a number of ovarian cancer cell lines and ovarian
Adam Rosenthal et al.
Biomaterials, 28(21), 3208-3216 (2007-04-17)
Cell-cell signaling is an important component of the stem cell microenvironment, affecting both differentiation and self-renewal. However, traditional cell-culture techniques do not provide precise control over cell-cell interactions, while existing cell-patterning technologies are limited when used with proliferating or motile

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