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Merck
CN

SAB4200692

Anti-Glutathione-S-Transferase (GST) antibody, Mouse monoclonal

clone GST-2, purified from hybridoma cell culture

别名:

GST, Glutathione-S-Transferase

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NACRES:
NA.46
UNSPSC Code:
12352203
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产品名称

Anti-Glutathione-S-Transferase (GST) antibody, Mouse monoclonal, clone GST-2, purified from hybridoma cell culture

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

GST-2, monoclonal

form

buffered aqueous solution

concentration

~1 mg/mL

technique(s)

ELISA: suitable
dot blot: suitable
immunoblotting: 0.5 μg/mL
immunofluorescence: suitable
immunoprecipitation (IP): suitable

isotype

IgG2b

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

Application

Anti-Glutathione-S-Transferase (GST) antibody, Mouse monoclonal has been used in:
  • immunoblotting
  • immunoprecipitation
  • dot blotting
  • autoradiography
  • immunofluorescence
  • enzyme-linked immunosorbent assay (ELISA)

Biochem/physiol Actions

Glutathione-S-Transferase (GST) helps to catalyze the conjugation of electrophilic substrates to reduced form of glutathione (GSH). Genetic engineering uses glutathione-S-transferase (from parasitic helminth Schistosoma japonicum) to create the GST gene fusion system for production of GST-labeled recombinant proteins. GST-fusion proteins were successfully demonstrated in structure- function studies involving protein-protein and DNA protein interactions via GST pull down assays.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Anti-Glutathione-S-Transferase (GST) antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the hybridoma GST-2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a purified recombinant GST fusion protein. Glutathione-S-Transferase (GST) is a 26 kDa protein found in eukaryotic cells. It belongs to the phase II detoxification enzymes family.

Immunogen

purified recombinant GST fusion protein

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

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存储类别

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品
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分析证书(COA)

Lot/Batch Number

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Viral pathogens hitchhike with insect sperm for paternal transmission
Mao Q, et al.
Nature Communications, 10(955), 1-10 (2019)
Manhui Xia et al.
OncoTargets and therapy, 12, 1575-1582 (2019-03-19)
Senescence has been recognized as a mechanism for the suppression of tumorigenesis. However, how the senescence is regulated is not fully understood. The present study aims to elucidate MLL3-mediated regulation of senescence. MLL3 protein levels in esophageal squamous cell carcinoma
MLL3 promotes the senescence of esophageal squamous cell carcinoma
Xia M, et al.
OncoTargets and Therapy, 12, 1575-1575 (2019)
Naveen Malik et al.
The Plant journal : for cell and molecular biology, 101(6), 1411-1429 (2019-11-09)
Mediator, a multisubunit co-activator complex, regulates transcription in eukaryotes and is involved in diverse processes in Arabidopsis through its different subunits. Here, we have explored developmental aspects of one of the rice Mediator subunit gene OsMED14_1. We analyzed its expression
Qianzhuo Mao et al.
Nature communications, 10(1), 955-955 (2019-03-01)
Arthropod-borne viruses (arboviruses) can be maternally transmitted by female insects to their offspring, however, it is unknown whether male sperm can directly interact with the arbovirus and mediate its paternal transmission. Here we report that an important rice arbovirus is

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