产品名称
Anti-Neurofilament 200 (Phos and Non-Phos) antibody, Mouse monoclonal, clone N52, purified from hybridoma cell culture
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
N52, monoclonal
mol wt
~200 kDa
species reactivity
mouse, pig, feline, rat, monkey, bovine, human
concentration
~1.0 mg/mL
technique(s)
immunoblotting: 2.5-5 μg/mL using human neuroblastoma SH-SY5Y cell line fresh lysate
immunofluorescence: suitable
immunohistochemistry: 10 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human Cerebellum sections
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
pig ... NEFH(100156492)
General description
Monoclonal Anti-Neurofilament 200 (phosphorylated and non-phosphorylated) (mouse IgG1 isotype) is derived from the N52 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Neurofilament 200 also known as Neurofilament heavy polypeptide (H-subunit) and is 200 kDa.
Neurofilament 200 is encoded by the gene located at human chromosome 22q12.2.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Application
Anti-Neurofilament 200 (Phos and Non-Phos) antibody has been used in
- immunoblotting
- immunofluorescence
- immunohistochemistry
Biochem/physiol Actions
Monoclonal Anti-Neurofilament 200 (Phos. and NonPhos.) also known as Neurofilament-H or Heavy subunit, specifically recognizes an epitope on the C-terminal tail domain of Neurofilament 200, which is present on both the phosphorylated and nonphosphorylated forms of this glycoprotein. The antibody shows reactivity with neurofilaments in the central and peripheral nervous systems from human , pig, mouse, rat, monkey, feline and bovine origin.
Mutation in the NEFH gene leads to the development of autosomal dominant axonal Charcot-Marie-Tooth disease (CMT2cc).
Neurofilament 200 has an important function in axonal transport, axonal plasticity and maintaining the structure of neuronal cytoskeleton. Defects in Neurofilament 200 are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) and these accumulations are a hallmark of pathological lesion.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
Preparation Note
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Dissociation of axonal neurofilament content from its transport rate
Yuan A, et al.
Testing, 10(7), e0133848-e0133848 (2015)
Natalia Dubrowinskaja et al.
Cancer medicine, 3(2), 300-309 (2014-01-28)
Neurofilament Heavy polypeptid (NEFH) belongs to the group of type IV intermediate filament proteins. DNA methylation of the NEFH promoter and loss of expression have previously been shown to activate the AKT/β-catenin pathway in tumor cells. When identifying hypermethylation of
Kenji Kashiwagi et al.
Investigative ophthalmology & visual science, 44(1), 154-159 (2002-12-31)
To investigate the phosphorylation of the heavy neurofilament subunit (NF-H), which could be deeply involved in axonal transport of retinal ganglion cells (RGCs), in an experimental glaucoma model of chronic elevation of intraocular pressure (IOP) in monkeys. One eye in
Alterations of a 200 kDa neurofilament in the rat hippocampus after forebrain ischemia
Kaku Y, et al.
Journal of Cerebral Blood Flow and Metabolism, 13(3), 402-408 (1993)
Jia-Hui Sun et al.
Neural regeneration research, 17(5), 1023-1033 (2021-09-25)
The formation of nerve bundles, which is partially regulated by neural cell adhesion molecule 1 (NCAM1), is important for neural network organization during peripheral nerve regeneration. However, little is known about how the extracellular matrix (ECM) microenvironment affects this process.
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