SAB4200752
Anti-Nuclease P1 antibody produced in rabbit
Ig fraction of antiserum, buffered aqueous solution
别名:
Anti-Deoxyribonuclease P1, Anti-Endonuclease P1
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352202
Clone:
polyclonal
Technique(s):
immunoblotting: 1:10,000-1:20,000 using Nuclease P1 from Penicillium citrinum
Application:
—
Citations:
3
biological source
rabbit
antibody form
Ig fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
~30 kDa
concentration
~1 mg/mL
technique(s)
immunoblotting: 1:10,000-1:20,000 using Nuclease P1 from Penicillium citrinum
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Nuclease P1 (Nuclease 5′-Nucleotidehydrolase, 3′- Phosphohydrolase, EC 3.1.30.1), also known as deoxyribonuclease P1 or endonuclease P1, is a zinc dependent endonuclease. It is isolated from Penicillium citrinum.
Immunogen
Nuclease P1 from Penicillium citrinum
Application
Anti-Nuclease P1 antibody produced in rabbit has been used in immunoblotting.
Biochem/physiol Actions
Nuclease P1 catalyzes the hydrolysis of 3′-5′ phosphodiester bonds in RNA and single-stranded DNA and 3′-phosphomonoester bonds in mono- and oligonucleotides terminated by a 3′-phosphate group without apparent bases specificity. The enzyme is used as a component of nuclease cocktail to generate single-stranded oligodeoxynucleotide (ODNs) samples for mass spectroscopy research for characterization of oligodeoxyribonucleotides. Nuclease P1 has also been widely used in the food industry to enhance or create flavor.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Production, purification and characterization of nuclease p1 from Penicillium citrinum
Guo-Qing Y, et al.
Process Biochemistry (Oxford, United Kingdom), 41(6), 1276-1281 (2006)
Nuclease digestion and mass spectrometric characterization of oligodeoxyribonucleotides containing 1, 2-GpG, 1, 2-ApG, and 1, 3-GpXpG cisplatin intrastrand cross-links
Williams RT, et al.
Clinica Chimica Acta; International Journal of Clinical Chemistry, 420(6), 160-170 (2013)
Identity of Phosphodiesterase and Phosphomonoesterase Activities with Nuclease P1 (a Nuclease from Penicillium citrinum)
Fujimoto, M., et al.
Agricultural and Biological Chemistry, 38, 785-790 (1974)
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