Monoclonal Anti-CD18, low endotoxin antibody produced in mouse

ITGB2 (integrin β2) encodes common β-chain of the β2 integrin family, known as cluster of differentiation 18 (CD18). CD18, integrin beta2 subunit, forms heterodimers with fourtypes of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2(CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2(CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediatedby the integrin is a composite of the functions of its individual subunits.These integrins are essential for proper leukocyte migration, mediatingintercellular contacts. Absence of CD18 leads to leukocyte adhesiondeficiency-1; severe reduction of CD18 expression leads to the development of apsoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella)haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediatedcytolysis. CD18 is primarily expressed in blood. The antibody MEM-148 recognizes an epitope on CD18 which is essentially inaccessible in intact integrin molecules on resting leukocytes, but is exposed on high-affinity state of LFA-1 or on unassociated CD18. CD18 (integrin beta2 subunit; beta2 integrin) is a 90-95 kDa type I transmembrane protein expressed on all leukocytes. In human chromosome, the gene CD18 is localized on 21q22.3.

Peripheral blood mononuclear cells

The antibody MEM-148 induces homotypic cell aggregation and high-affinity LFA-1 conformation in resting leukocytes. Flow cytometry: Recommended dilution: 8 μg/ml. The antibody MEM-148 is an excellent marker of activated myeloid cells. Western blotting: Non-reducing conditions

Cluster of differentiation (CD18) is involved in adhesion of leukocytes to the endothelium and its migration to the site of inflammation. Lower expression of CD18 is critical because of its involvement in all the heterodimeric complex and controls the expression of α subunit. CD18 is a part of intracellular signalling molecule, signals through phosphatidylinositol 3 kinase (PI3K) and tyrosine kinase. Mutations in ITGB2 causes autosomal recessive leukocyte adhesion deficiency type 1 disorder, characterized by frequent bacterial and fungal infections. Polymorphism in ITGB2 is associated with chronic lymphocytic leukemia (CLL). Mutations in CD18 causes Hirschsprung′s disease (HSCR)-associated enterocolitis (HAEC) and chronic colitis. ITGB2 is upregulated in atherosclerotic plaque.

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Solution in azide free phosphate buffered saline, pH 7.4; 0.2 um filter sterilized. Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.