生物来源
alpaca
质量水平
重组
expressed in Pichia pastoris
偶联物
agarose conjugate
抗体产品类型
primary antibodies
克隆
recombinant monoclonal
表单
liquid
分子量
15 kDa
技术
ChIP: suitable
RIP: suitable
co-immunoprecipitation (co-IP): suitable
immunoprecipitation (IP): suitable
mass spectrometry (MS): suitable
颜色
colorless
基质
(~45 um (cross-linked to 7.5% agarose beads))
同位素/亚型
VHH
容量
binding capacity (10 μL slurry binds about 3ug-4 μg of recombinant GFP.)
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
一般描述
Fluorescent protein GFP exhibits bright green fluorescence when exposed to ultraviolet light and is frequently used as reporter and fusion tag. Anti-GFP single domain antibody magnetic beads are magnetic agarose spheres covalently coupled with VHH antibodies acquiring high specificity and affinity for GFP. These beads can efficiently capture and separate GFP and GFP tagged proteins, alongside with the associated proteins from cell extracts of mammal, plant, bacteria, yeast, insect, and other organisms.
免疫原
GFP Tagged Protein
生化/生理作用
Selectively recognizes GFP, mEGFP, superfolder GFP and most common CFP and YFP variants.
特点和优势
- Anti Tag-Nano antibody beads are single-domain/variable region of heavy chain of camelid antibodies(sdAb/VHH).
- They are directly coupled to beads covalently.
- They have unique properties such as small size (~15kDa, 2-4nm) when compared to conventional antibodies.
- No heavy and light chains in downstream interactions.
- Acids and alkali resistance, tolerance to harsh conditions.
- Short incubation time 5 – 30 minutes.
- Low batch to batch variations.
- Typically, antibodies are not detectable in Western Blot.
包装
plastic tube
外形
1XPBS, 0.03% sodium azide, 50% glycerol.
其他说明
Before usage, please invert the vial several times (DO NOT VORTEX) to form beads suspension, and take out beads’ suspension using a pipette tip with bigger opening (i.e., cut the tip of a 1000 μl pipette tip with sterile scissors). Once opened, it is recommended to use paraffin film to seal the cap.
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