biological source
alpaca
recombinant
expressed in Pichia pastoris
conjugate
magnetic beads conjugate
antibody product type
primary antibodies
clone
recombinant monoclonal
form
liquid
mol wt
15 kDa
technique(s)
ChIP: suitable, RIP: suitable, co-immunoprecipitation (co-IP): suitable, immunoprecipitation (IP): suitable, mass spectrometry (MS): suitable
color
black
matrix
(~30 -100um magnetic agarose beads)
isotype
VHH
capacity
binding capacity(10 μL slurry binds about 3ug-4μg of recombinant GFP.)
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
General description
Fluorescent protein GFP exhibits bright green fluorescence when exposed to ultraviolet light and is frequently used as reporter and fusion tag. Anti-GFP single domain antibody agarose beads are agarose beads covalently coupled with VHH antibodies acquiring high specificity and affinity for GFP. These beads can efficiently capture and separate GFP and GFP tagged proteins, alongside with the associated proteins from cell extracts of mammal, plant, bacteria, yeast, insect, and other organisms.
Immunogen
GFP Tagged Protein
Biochem/physiol Actions
Selectively recognizes GFP, mEGFP, superfolder GFP and most common CFP and YFP variants, do not cross-react with mCherry, mRFP, dsRed, mTagBFP, mTagRFP or their most common derivatives.
Features and Benefits
- Anti Tag-Nano antibody beads are single-domain/variable region of heavy chain of camelid antibodies(sdAb/VHH).
- They are directly coupled to beads covalently.
- They have unique properties such as small size (~15kDa, 2-4nm) when compared to conventional antibodies.
- No heavy and light chains in downstream interactions.
- Acids and alkali resistance, tolerance to harsh conditions.
- Short incubation time 5 – 30 minutes.
- Low batch to batch variations.
- Typically, antibodies are not detectable in Western Blot.
Packaging
plastic tube
Physical form
1XPBS, 0.03% sodium azide, 50% glycerol.
Other Notes
Before usage, please invert the vial several times (DO NOT VORTEX) to form beads suspension, and take out beads’ suspension using a pipette tip with bigger opening (i.e., cut the tip of a 1000 μl pipette tip with sterile scissors). Once opened, it is recommended to use paraffin film to seal the cap.
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