biological source
rabbit (Monoclonal)
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
23GB6055, recombinant monoclonal
form
buffered aqueous glycerol solution
mol wt
11 kDa
species reactivity
rat, human
packaging
vial of 1.5 mL
enhanced validation
recombinant expression
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Quality Level
Gene Information
human ... H4C3(8364)
sustainability
Greener Alternative Product
concentration
0.4 mg/ml
technique(s)
flow cytometry: 1:2000, immunofluorescence: 1:100-:1000, western blot: 1:1,000-1:5,000
isotype
IgG
Ensembl | human accession no.
NCBI accession no.
UniProt accession no.
greener alternative category
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]
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Immunogen
A synthesized peptide corresponding to a sequence within amino acids 1-13 of human Histone H4 (acetyl K5) [NP_001029249.1].
Physical form
Solution in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Other Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
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