SRP0188
Pyruvate kinase M2 Active human
recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
别名:
M2-PK tumor, OPA-interacting protein 3 (OIP-3), Pyruvate kinase 3 (PK3), Pyruvate kinase, muscle, Thyroid hormone-binding protein 1 (THBP1)
biological source
human
recombinant
expressed in E. coli
assay
≥90% (SDS-PAGE)
form
aqueous solution
mol wt
58.9 kDa
storage condition
avoid repeated freeze/thaw cycles
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PKM2(5315)
General description
Human Pyruvate kinase M2 (PKM2) (GenBank Accession No. NM_002654) full length with N-terminal His tag, MW = 58.9 kDa, expressed in an E. coli expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Biochem/physiol Actions
Pyruvate kinase M2 (PKM2) is an isoform of pyruvate kinase that is expressed in certain differentiated cells and in proliferatively active cells. PKM2 is often overexpressed in cancer cells.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Other Notes
One unit is defined as the amount of enzyme required to transfer a phosphoryl group from phosphoenolpyruvate (PEP) to 1 pmol of ADP/min at 37°C
法规信息
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Chompoonuch Sawaengsak et al.
International journal of pharmaceutics, 473(1-2), 113-125 (2014-07-08)
An egg-free and broadly effective influenza vaccine that can be produced rapidly, adequately and cost-effectively is needed. In this study, chitosan-associated DNAs prepared at various nitrogen/phosphate charge (N/P) ratios were studied for their physicochemical properties, stability, cytotoxicity, and protein expression
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