产品名称
Bas Active human, recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
biological source
human
recombinant
expressed in E. coli
assay
≥80% (SDS-PAGE)
form
aqueous solution
mol wt
71.8 kDa
packaging
pkg of 20 μg
storage condition
avoid repeated freeze/thaw cycles
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PTPN13(5783)
Application
Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.
General description
Protein tyrosine phosphatase. Catalytic domain, (amino acids 2091-2490), (Gene bank accession number NM_080685) with N-terminal GST tag, MW = 71.8 kDa, expressed in an E. coli expression system.
Other Notes
One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
Physical form
Formulation: recombinant proteins were eluted in 25 mM Tris-HCL, 75 mM NaCl, pH 8.0, 0.05% Tween, 10 mM glutathione, 1 mM DTT and 50% glycerol.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
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