SRP0278
PDE11A4 Active human
recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)
别名:
PPNAD2, Phosphodiesterase 11A4, cAMP and cGMP cyclic nucleotide phosphodiesterase 11A
biological source
human
recombinant
expressed in baculovirus infected insect cells
assay
≥80% (SDS-PAGE)
form
aqueous solution
mol wt
131 kDa
packaging
pkg of 10 μg
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PDE11A(50940)
General description
PDE11A (phosphodiesterase 11A) belongs to the PDE protein family, and is expressed in three isoforms. Its mRNA has been found to be expressed in skeletal muscle, prostate, kidney, liver, pituitary, and salivary glands and testis. All PDEs contain a conserved PDE catalytic domain at the C-terminal, which also contains the PDE signature motif HDX2HX4N. This gene is localized to human chromosome 2q31–2q35.
Human PDE11A4 (GenBank Accession No. BAB62712), full length with N-terminal GST tag, MW = 131kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Human PDE11A4 (GenBank Accession No. BAB62712), full length with N-terminal GST tag, MW = 131kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Biochem/physiol Actions
Phosphodiesterases (PDEs) function as regulators of cyclic nucleotide levels. PDE11A is a dual-specificity PDE that might be involved in controlling both cGMP and cAMP levels under physiological conditions. A genome-wide study shows that the inactivation of this gene is linked with adrenocortical hyperplasia. Polymorphisms in this gene are also linked with ACTH (adrenocorticotropic hormone)-independent macronodular adrenal hyperplasia (AIMAH). PDE11A is also involved in the control of cortisol secretion. In Carney complex (CNC) patients with germline PRKAR1A mutations, polymorphisms in PDE11A might influence the development of testicular and adrenal tumors by acting as a modifier of the phenotype.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, 5 mM glutathione and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Other Notes
One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′ AMP per min at 37°C
法规信息
新产品
此项目有
A genome-wide scan identifies mutations in the gene encoding phosphodiesterase 11A4 (PDE11A) in individuals with adrenocortical hyperplasia.
Horvath A et al
Nature Genetics, 38(7), 794-800 (2006)
Phosphodiesterase 11A (PDE11A) gene defects in patients with acth-independent macronodular adrenal hyperplasia (AIMAH): functional variants may contribute to genetic susceptibility of bilateral adrenal tumors.
Vezzosi D et al
The Journal of Clinical Endocrinology and Metabolism, 97(11), E2063-E2069 (2012)
Frequent phosphodiesterase 11A gene (PDE11A) defects in patients with Carney complex (CNC) caused by PRKAR1A mutations: PDE11A may contribute to adrenal and testicular tumors in CNC as a modifier of the phenotype.
Libe R et al
The Journal of Clinical Endocrinology and Metabolism, 96(1), E208-E214 (2011)
Molecular cloning and characterization of a distinct human phosphodiesterase gene family: PDE11A.
Fawcett L et al
Proceedings of the National Academy of Sciences of the USA, 97(7), 3702-3707 (2000)
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持