SRP4015
CD40Ligand/TRAP from mouse
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)
别名:
CD154 antigen, CD40-L, T cell antigen Gp39, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5
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NACRES:
NA.32
UNSPSC Code:
12352200
Form:
lyophilized
Assay:
≥98% (HPLC), ≥98% (SDS-PAGE)
Biological source:
mouse
Recombinant:
expressed in E. coli
Mol wt:
~16.4 kDa
biological source
mouse
recombinant
expressed in E. coli
assay
≥98% (HPLC), ≥98% (SDS-PAGE)
form
lyophilized
potency
<0.1 μg/mL ED50
mol wt
~16.4 kDa
packaging
pkg of 25 μg
storage condition
avoid repeated freeze/thaw cycles
impurities
endotoxin, tested
NCBI accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
mouse ... Cd40lg(21947)
General description
CD40 Ligand (CD40L/TRAP) is a type II transmembrane glycoprotein belonging to the TNF superfamily of ligands. Natually, CD40L is a membrane-bound protein, but proteolytically cleaved 15-18 kDa soluble forms of CD40L are also fully active. CD40L plays an important role in the proliferation and differentiation of B-cells, and is critical for immunoglobulin (Ig) class switching. Recombinant mouse CD40 Ligand is a soluble 18 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CDE40L.
Physical form
Sterile filtered and then lyophilized from 10 mM Sodium Phosphate, pH 7.5 plus 0.1 M Arginine.
Preparation Note
Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.
Reconstitute in water to a concentration of 0.1-1.0 mg/mL. The solution can then be diluted into other aqueous buffers.
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