Apo-D human

Apolipoprotein D (APOD) plays an essential role in the development and repair of the brain and protection from oxidative stress. In addition, it also plays an essential role in lipid metabolism by facilitating the binding of HDL (high density lipoprotein) to LDL (low density lipoprotein) and to grow T24 carcinomas. Variation in the APOD expression acts as a genetic marker for obesity and several components of syndrome X.

Apolipoprotein D (APOD) is encoded by the gene mapped to human chromosome 3q29. The encoded protein is ubiquitously expressed in the body, but at high levels in glial cells of the brain, fibroblasts, spleen, testes and mammary cysts. APOD is a lipocalin containing an eight-stranded antiparallel β-sheet barrel-like structure forming a hydrophobic pocket.

Apolipoprotein-D (Apo-D) is mainly associated with high density lipoproteins in human plasma. It is expressed in numerous tissues having high levels of expression in spleen, testes and brain. Apo-D is a multi-ligand, multi-functional transporter and transports a ligand from 1 cell to another within an organ, scavenge a ligand within an organ for transport to the blood or could transport a ligand from the circulation to specific cells within a tissue. The recombinant human Apo-D expressed from E. coli is a single, non-glycosylated, Polypeptide chain containing 174 amino acids and having a molecular mass of 19.8 kDa.

Lyophilized from 1 mg/ml in 4 mM KH2PO4, 16 mM Na2HPO4 and 115 mM NaCl pH7.5.

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in dH₂O to a working volume of 0.5 mg/mL and let the lyophilized pellet dissolve completely. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.