biological source
human
recombinant
expressed in E. coli
assay
≥98% (HPLC), ≥98% (SDS-PAGE)
form
lyophilized
mol wt
~31.2 kDa
packaging
pkg of 20 μg
impurities
endotoxin, tested
NCBI accession no.
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... GDF9(2661)
General description
GDF-9 is a member of the TGF-β superfamily of growth and differentiation factors, and is highly homologous to GDF-3. Unlike most TGF-family members, GDF-9 and GDF-3 are not disulfide-linked dimers. GDF-3 is expressed in adult bone marrow, spleen, thymus, and adipose tissue. GDF-9 is expressed in oocytes and is required for normal ovarian folliculogenesis. Recombinant human GDF-9 is a 31.0 kDa non-disulfide-linked homodimer containing two 135 amino acid polypeptide chains with 6XHis on its N-terminus.
Application
GDF9 has been as a supplement in the basal medium for the maturation of cumulus–oocyte complexes (COCs).
Biochem/physiol Actions
Growth differentiation factor 9 (GDF9) is known to be localized in the cytoplasm. The gene encoding it is localized on human chromosome 5q31.1. It is known to be associated with granulosa cell proliferation and steroid production. Downregulation of the gene is observed in polycystic ovary syndrome.
Physical form
Sterile filtered and lyophilized with no additives.
Preparation Note
Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.
Reconstitute in water to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
涉药品监管产品
此项目有
Impact of oocyte-secreted factors on its developmental competence in buffalo
Swati Gupta
Zygote (2017)
Signalling pathways involved in the synergistic effects of human growth differentiation factor 9 and bone morphogenetic protein 15.
Reader KL
Reproduction, Fertility, and Development (2016)
Mutations and sequence variants in GDF9 and BMP15 in patients with premature ovarian failure.
Laissue P
European Journal of Endocrinology (2006)
Reduced and delayed expression of GDF9 and BMP15 in ovarian tissues from women with polycystic ovary syndrome.
Wei LN
Journal of Assisted Reproduction and Genetics (2014)
Fang-Ju Wu et al.
Molecular and cellular endocrinology, 522, 111121-111121 (2020-12-19)
Cumulus expansion is essential for ovulation and oocyte maturation in mammals. Previous studies suggest that this process requires certain cumulus expansion enabling factors, induced by LH surge, that activate SMAD signaling locally. However, their identities remain uncertain. Using a superovulated
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