生物来源
human
重组
expressed in E. coli
方案
≥98% (HPLC)
≥98% (SDS-PAGE)
表单
lyophilized
分子量
~31.2 kDa
包装
pkg of 20 μg
杂质
endotoxin, tested
NCBI登记号
UniProt登记号
运输
wet ice
储存温度
−20°C
基因信息
human ... GDF9(2661)
一般描述
GDF-9 is a member of the TGF-β superfamily of growth and differentiation factors, and is highly homologous to GDF-3. Unlike most TGF-family members, GDF-9 and GDF-3 are not disulfide-linked dimers. GDF-3 is expressed in adult bone marrow, spleen, thymus, and adipose tissue. GDF-9 is expressed in oocytes and is required for normal ovarian folliculogenesis. Recombinant human GDF-9 is a 31.0 kDa non-disulfide-linked homodimer containing two 135 amino acid polypeptide chains with 6XHis on its N-terminus.
应用
GDF9 has been as a supplement in the basal medium for the maturation of cumulus–oocyte complexes (COCs).
生化/生理作用
Growth differentiation factor 9 (GDF9) is known to be localized in the cytoplasm. The gene encoding it is localized on human chromosome 5q31.1. It is known to be associated with granulosa cell proliferation and steroid production. Downregulation of the gene is observed in polycystic ovary syndrome.
外形
Sterile filtered and lyophilized with no additives.
制备说明
Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.
Reconstitute in water to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers.
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
涉药品监管产品
此项目有
Signalling pathways involved in the synergistic effects of human growth differentiation factor 9 and bone morphogenetic protein 15.
Reader KL
Reproduction, Fertility, and Development (2016)
Impact of oocyte-secreted factors on its developmental competence in buffalo
Swati Gupta
Zygote (2017)
Reduced and delayed expression of GDF9 and BMP15 in ovarian tissues from women with polycystic ovary syndrome.
Wei LN
Journal of Assisted Reproduction and Genetics (2014)
Jesús Cadenas et al.
Reproductive biology and endocrinology : RB&E, 20(1), 126-126 (2022-08-20)
The suggested effects of the oocyte secreted GDF9 and BMP15 growth factors on oocyte maturation are currently based on recombinant proteins, and little is known about native GDF9 and BMP15 in humans. Human immature cumulus-oocyte complexes (COCs) obtained in connection
Mutations and sequence variants in GDF9 and BMP15 in patients with premature ovarian failure.
Laissue P
European Journal of Endocrinology (2006)
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