recombinant
expressed in E. coli
assay
≥95% (SDS-PAGE)
form
lyophilized
mol wt
~8.0 kDa
packaging
pkg of 10 μg
impurities
endotoxin, tested
NCBI accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
mouse ... Nucb2(53322)
General description
Nesfatin-1, a polypeptide produced by cleavage of the NEFA/nucleobindin-2 (NUCB2), is a secreted protein, expressed in the appetite-control hypothalamic nuclei. Nesfatin is involved in the regulation of the feeding behavior. Intracerebroventicular injection of nesfatin-1 reduces body weight in rats be affecting the animal food intake.
Physical form
Lyophilized from PBS.
Preparation Note
Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.
Reconstitute with sterile water to a concentration of 0.1-1 mg/mL. Further dilutions should be made with medium containing 0.2% BSA.
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