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Merck
CN

SRP4969

Epigen (EPGN) human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE)

别名:

ALGV3072, EPG, EPGN, Epigen, FLJ75542, PRO9904

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关于此项目

NACRES:
NA.32
UNSPSC Code:
12352202
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biological source

human

recombinant

expressed in E. coli

assay

≥98% (SDS-PAGE)

form

lyophilized

potency

150-300 ng/mL

mol wt

~7.9 kDa

packaging

pkg of 25 μg

impurities

endotoxin, tested

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... EPGN(255324)

General description

The precursor EPGN (epigen) is a transmembrane glycoprotein and is widely expressed in human tissues. The precursor is cleaved to give a soluble EGF (epidermal growth factor)-like domain.
Recombinant Human epigen, produced in Escherichia coli is a single, non-glycosylated, polypeptide chain containing 72 amino acids and having a molecular mass of 7.9kDa.

Biochem/physiol Actions

EPGN (epigen) is a ligand for EGFR (epidermal growth factor receptor). EPGN (epigen) belongs to the EGF family and enhances proliferation in keratinocytes.

Physical form

EPGN was lyophilized from 20 mM PBS buffer pH-7.4 containing 130 mM sodium chloride.

Preparation Note

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.
Reconstitute in sterile d-H2O not less than 100 μg/ml. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA).

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Fetal inhibition of inflammation improves disease phenotypes in harlequin ichthyosis.
Cottle DL, et al.
Human Molecular Genetics, 24, 436-436 (2015)
Epigen, the last ligand of ErbB receptors, reveals intricate relationships between affinity and mitogenicity.
Kochupurakkal BS, et al.
The Journal of Biological Chemistry, 280, 8503-8503 (2005)
The EGFR-HER2 module: a stem cell approach to understanding a prime target and driver of solid tumors.
Schneider MR and Yarden Y
Oncogene, 35, 2949-2949 (2019)

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