SRP5051
NEK11, active, GST tagged human
PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
别名:
FLJ23495
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关于此项目
NACRES:
NA.32
UNSPSC Code:
12352200
Form:
buffered aqueous glycerol solution
Assay:
≥70% (SDS-PAGE)
Recombinant:
expressed in baculovirus infected Sf9 cells
Mol wt:
~110 kDa
recombinant
expressed in baculovirus infected Sf9 cells
product line
PRECISIO® Kinase
assay
≥70% (SDS-PAGE)
form
buffered aqueous glycerol solution
specific activity
21-29 nmol/min·mg
mol wt
~110 kDa
NCBI accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... NEK11(79858)
General description
NEK11 is a member of the NEK family and the protein localizes to the nucleoli where it may function with NEK2A in the S-phase checkpoint. NEK2 phosphorylates NEK11 in its C-terminal autoinhibitory domain and elevates NEK11 kinase activity by dissociating the autoinhibitory domain from the N-terminal catalytic domain. NEK11 protein appears to play a role in DNA replication and response to genotoxic stress. NEK11 activity is activated in HeLa cells treated with various DNA-damaging agents and replication inhibitors. Activation of NEK11 by genotoxic agents is suppressed by inhibitor of ATR/ATM-mediated signalling indicating that NEK11 has a role in S-phase checkpoint downstream of ATR/ATM signalling.
Physical form
Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation Note
after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable