SRP5095
TTK，活性，GST标记 人
PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
别名:
ESK, FLJ38280, MPS1, MPS1L1, PYT
General description
TTK是一种丝氨酸/苏氨酸激酶，与中心体复制和有丝分裂检查点反应的调节有关。显性阴性TTK在人细胞系中的过表达可防止中心体复制，而活性TTK则可加速骨肉瘤细胞系中的中心体复制。TTK功能的破坏导致严重的有丝分裂异常和中心体复制失败。在纺锤体破坏过程中，稳定和激活p53需要TTK。TTK磷酸化Thr18处p53的N末端结构域，这种磷酸化破坏了与MDM2的相互作用，并消除了MDM2介导的p53泛素化。
Physical form
以 50 mM Tris-HCl，pH 7.5，150mM NaCl，10 mM 谷胱甘肽，0.1 mM EDTA，0.25 mM DTT，0.1 mM PMSF，25％ 甘油的形式提供。
Preparation Note
开封后，分装少量，-70℃ 储存。避免重复处理和多次冻融循环
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
10 - Combustible liquids
wgk
WGK 1
法规信息
常规特殊物品
此项目有
Yi-Fu Huang et al.
Molecular and cellular biology, 29(11), 2935-2944 (2009-04-01)
Upon prolonged arrest in mitosis, cells undergo adaptation and exit mitosis without cell division. These tetraploid cells are either eliminated by apoptosis or arrested in the subsequent G(1) phase in a spindle checkpoint- and p53-dependent manner. p53 has long been
Harold A Fisk et al.
Proceedings of the National Academy of Sciences of the United States of America, 100(25), 14875-14880 (2003-12-06)
The mitotic spindle is essential for the maintenance of genetic stability, and in budding yeast its assembly and function depend on the Mps1 protein kinase. Mps1p is required for centrosome duplication and the spindle checkpoint. Several recent reports demonstrate that
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