biological source
mouse
recombinant
expressed in E. coli
assay
≥97% (HPLC), ≥97% (SDS-PAGE)
form
lyophilized
mol wt
19.8 kDa
impurities
Endotoxin, tested
NCBI accession no.
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
mouse ... SHH(20423)
General description
Sonic hedgehog (Shh) is a morphogenic glycoprotein. It acts as an activator of embryonic development. Three mammalian hedgehog genes (sonic, desert, Indian) share about 60% homology. The Mouse Sonic Hedgehog is 99% homologous to the human gene. Cysteine at position 25 has been substituted with isoleucine. Mouse SHH is a non-glycosylated polypeptide consisting of 176 amino acids with a molecular weight of 19,800 Da. The gene encoding Shh is localized on mouse chromosome 5.
Application
Sonic Hedgehog from mouse has been used for gallbladder organ culture.
Biochem/physiol Actions
Shh is activated in osteoblasts and has a role in fracture healing and regeneration. The protein associates with its receptor Patched (Ptc-1), de-represses Smoothened (Smo) and activates the Gli family of transcription factors. It has a role in cell differentiation, proliferation, migration and survival. Mutations in the Shh pathway have been linked to developmental diseases and cancer.
Physical form
Lyophilized from a 10 mM sodium phosphate buffer, pH 7.5.
Preparation Note
Centrifuge the vial prior to opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile H?O to a concentration of 0.1-0.5 mg/ml, which can be further diluted into other aqueous solutions.
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Embryonic Cholecystitis and Defective Gallbladder Contraction in the
Sox17-haploinsufficient Model of Biliary Atresia
Sox17-haploinsufficient Model of Biliary Atresia
Hiroki H
Developmental Biology (2017)
Woo Jean Kim et al.
International journal of molecular medicine, 34(1), 213-218 (2014-05-09)
Lately, the importance of the communication between different cell types and the understanding of the cell communication pathway has been emphasized, as it may provide a novel therapeutic strategy for the regeneration of damaged tissue. In the present study, we
Cloning, expression, and chromosomal location of SHH and IHH: two human homologues of the Drosophila segment polarity gene hedgehog.
Marigo V
Genomics, 28(1), 44-51 (1995)
Tian Lan et al.
PloS one, 10(7), e0129743-e0129743 (2015-07-30)
Reactive oxygen species (ROS) produced by nicotinamide adenine dinucleotide phosphate oxidase (NOX) play a key role in liver injury and fibrosis. Previous studies demonstrated that GKT137831, a dual NOX1/4 inhibitor, attenuated liver fibrosis in mice as well as pro-fibrotic genes
Sonic hedgehog stimulates neurite outgrowth in a mechanical stretch model of reactive-astrogliosis.
Berretta A
Scientific Reports, 6, 21896-21896 (2016)
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