Sonic Hedgehog from mouse

Sonic hedgehog (Shh) is a morphogenic glycoprotein. It acts as an activator of embryonic development. Three mammalian hedgehog genes (sonic, desert, Indian) share about 60% homology. The Mouse Sonic Hedgehog is 99% homologous to the human gene. Cysteine at position 25 has been substituted with isoleucine. Mouse SHH is a non-glycosylated polypeptide consisting of 176 amino acids with a molecular weight of 19,800 Da. The gene encoding Shh is localized on mouse chromosome 5.

Sonic Hedgehog from mouse has been used for gallbladder organ culture.

Shh is activated in osteoblasts and has a role in fracture healing and regeneration. The protein associates with its receptor Patched (Ptc-1), de-represses Smoothened (Smo) and activates the Gli family of transcription factors. It has a role in cell differentiation, proliferation, migration and survival. Mutations in the Shh pathway have been linked to developmental diseases and cancer.

Lyophilized from a 10 mM sodium phosphate buffer, pH 7.5.

Centrifuge the vial prior to opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile H?O to a concentration of 0.1-0.5 mg/ml, which can be further diluted into other aqueous solutions.