SRP6266
肾素 来源于小鼠
recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)
别名:
CYM, CYMP, RENNIN, 凝乳酶
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关于此项目
NACRES:
NA.32
UNSPSC Code:
12352200
Form:
lyophilized powder
Assay:
≥95% (SDS-PAGE)
Biological source:
mouse
Recombinant:
expressed in HEK 293 cells
Mol wt:
calculated mol wt 43.2 kDa, observed mol wt 45-55 kDa by SDS-PAGE (reducing) (Protein migrates due to glycosylation. Leu24 is the predicted N-terminus.)
biological source
mouse
recombinant
expressed in HEK 293 cells
tag
6-His tagged (C-terminus)
assay
≥95% (SDS-PAGE)
form
lyophilized powder
mol wt
calculated mol wt 43.2 kDa, observed mol wt 45-55 kDa by SDS-PAGE (reducing) (Protein migrates due to glycosylation. Leu24 is the predicted N-terminus.)
packaging
pkg of 10 μg
impurities
<1 EU/μg endotoxin (LAL test)
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
mouse ... REN(19701)
General description
肾素，也称为 REN 和血管紧张素原酶，是一种循环酶，参与人体′的肾素-血管紧张素系统（RAS）。编码它的基因位于小鼠 1 号染色体上。
Biochem/physiol Actions
肾素在动脉血压升高和肾钠潴留增加中起重要作用。它通过裂解肝脏产生的血管紧张素原来激活肾素-血管紧张素系统，产生血管紧张素 I，它进一步通过血管紧张素转换酶（ACE）转化为血管紧张素 II，血管紧张素转换酶主要在肺毛细血管内。肾素为肾细胞所分泌，该分泌作用由通过致密斑（响应通过远端肾小管的液体流速）的信号而被激活，该信号是通过降低肾脏灌注压力（通过血管壁中的牵张感受器），以及通过交感神经刺激（主要是通过 β-1 受体激活）引起的。它可以与 ATPase H+ 转运辅助蛋白 2（ATP6AP2）结合，从而使血管紧张素原转化为血管紧张素 I 的转化率比可溶性肾素高四倍。此外，它的结合会导致 ATP6AP2 的丝氨酸和酪氨酸残基磷酸化。肾素 mRNA 的水平似乎受到羟酰基辅酶 A 脱氢酶/3-酮酰基辅酶 A 硫解酶/烯酰辅酶 A 水合酶（三功能蛋白）、β 亚基（HADHB）、HuR 和 CP1 与 3′ UTR 调控区结合的调控。肾素-血管紧张素系统过度活跃会导致血管收缩以及钠和水的滞留。这些作用导致高血压。因此，肾素抑制剂可用于治疗高血压。
Physical form
从 0.22 μm 过滤的 PBS 溶液中冻干。冻干前一般加入5-8%甘露醇或海藻糖作为保护剂。
Preparation Note
打开样品瓶之前需离心。用无菌的PBS，pH7.4重悬成浓度为50 μg/mL的溶液。不可涡旋。该溶液可在2-8°C下储存长达1个月。如要长期储存，建议将其存放于-20°C。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Classical Renin-Angiotensin system in kidney physiology.
Sparks MA
Comprehensive Physiology, 4(3), 1201-1228 (2014)
Regulated expression of the Ren-2 gene in transgenic mice derived from parental strains carrying only the Ren-1 gene.
Tronik D
The Embo Journal, 6(4), 983-987 (1987)
Hsin-Chieh Lin et al.
Food & nutrition research, 61(1), 1391666-1391666 (2017-11-21)
Proteins from tilapia frame and skin can potentially be precursors of antihypertensive peptides according to the result of BIOPEP analyses. The aim was to generate peptides with inhibitory effects against angiotensin-converting enzyme (ACE) and renin from tilapia frame and skin protein
Renin: origin, secretion and synthesis.
Persson PB
The Journal of Physiology, 552(Pt 3), 667-671 (2003)
Yu Fu et al.
International journal of biological macromolecules, 101, 207-213 (2017-03-17)
In this work, the inhibitory effects of potato patatin-derived peptides Trp-Gly (WG) and Pro-Arg-Tyr (PRY) on angiotensin I-converting enzyme (ACE) and renin activities were investigated using kinetics, intrinsic fluorescence and molecular docking. The results indicated that PRY was a more
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